Process technology for biological product manufacturing and downstream purification

Abstract

Provided herein are, inter alia, biological manufacturing and downstream purification processes.

What is claimed is: 
     
         1 . A dynamic filtration apparatus for removing impurities from a biological product in a heterogeneous mixture, comprising:
 a filter membrane extending between a feed reel and a collection reel, the filter membrane having a target region that is configured to receive the heterogeneous mixture from at least one output head configured to dispense the heterogeneous mixture onto the target region;   a membrane support structure to structurally support a portion of the filter membrane that is positioned between the feed reel and the collection reel to create the target region;   at least one support member to stabilize the transport of the filter membrane across the membrane support structure;   a vacuum system configured to apply negative gauge pressure across the dynamic filter membrane.   
     
     
         2 . The apparatus of  claim 1 , further comprising a wash buffer line. 
     
     
         3 . The apparatus of  claim 1 , wherein the filter membrane comprises polyethersulfone (PES), hydrophilic polysulfone, cellulose ester, cellulose acetate, polyvinylidene fluoride (PVDF), hydrophilic PVDF, polycarbonate, nylon, polytetrafluoroethylene (PTFE), hydrophilic PTFE, or any combination thereof. 
     
     
         4 . The apparatus of  claim 1 , wherein the filter membrane comprises a pore size in the range from about 0.1 μm to about 1 μm. 
     
     
         5 . The apparatus of  claim 1 , wherein the membrane support structure includes a series of parallel slots. 
     
     
         6 . The apparatus of  claim 1 , wherein the gauge pressure ranges from about −0.05 bar to about −0.98 bar. 
     
     
         7 . The apparatus of  claim 1 , wherein the membrane support structure has a substantially smooth contact surface. 
     
     
         8 . The apparatus of  claim 1 , wherein the support member has a substantially smooth contact surface. 
     
     
         9 . The apparatus of  claim 7 , wherein the substantially smooth contact surface has a static coefficient of friction from about 0.01 to about 0.1. 
     
     
         10 . The apparatus of  claim 8 , wherein the substantially smooth contact surface has a static coefficient of friction from about 0.01 to about 0.1. 
     
     
         11 . The apparatus of  claim 1 , wherein the vacuum system comprises at least one vacuum line in communication with the membrane support structure. 
     
     
         12 . The apparatus of  claim 1 , wherein the apparatus further comprises a system configured to control the transport velocity of the filter membrane. 
     
     
         13 . The apparatus of  claim 12 , wherein the system comprises at least one motor. 
     
     
         14 . The apparatus of  claim 13 , wherein the motor is controlled by a closed-loop controller that operates a feedback mechanism. 
     
     
         15 . Use of the apparatus of  claim 1 , to purify a biological product from a heterogeneous mixture.