Hyaluronic acid derivative and use thereof

Abstract

The present invention provides a hydrogel platform using, as a substrate, hyaluronic acid (HA) conjugated to a pyrogallol (PG) moiety. The HA-PG conjugate of the present invention can be rapidly crosslinked by two different methods, in each of which an oxidizing agent is used or a pH is adjusted. The hydrogel of the present invention can not only have excellent biocompatibility, but also can have efficiently controlled physical characteristics such as a crosslinking rate, elasticity, and adhesive strength, depending on each crosslinking method. On the basis of such excellent stability and functionality, the hydrogel of the present invention can be used in various fields including drug delivery, biopharmaceutical materials such as a wound healing agent or anti-adhesive agent, medicines, and cosmetic products.

1 . A hyaluronic acid hydrogel comprising a plurality of hyaluronic acid derivative represented by the following Formula 5 as a repeating unit, wherein crosslinking represented by the following Formula 2 or Formula 3 is formed between the hyaluronic acid derivatives: 
       
         
           
           
               
               
           
         
         wherein HA′ represents hyaluronic acid in which the carboxyl group is substituted with an amide group. 
       
     
     
         2 . The hyaluronic acid hydrogel of  claim 1 , wherein the hyaluronic acid derivative has a molecular weight of 10,000 Da to 2,000,000 Da. 
     
     
         3 . The hyaluronic acid hydrogel of  claim 1 , wherein the hyaluronic acid derivative has a gallol group substitution rate of about 0.1% to 50%. 
     
     
         4 . A scaffold for tissue engineering, the scaffold comprising the hyaluronic acid hydrogel of  claim 1 . 
     
     
         5 . A drug delivery carrier comprising the hyaluronic acid hydrogel of  claim 1 . 
     
     
         6 . The drug delivery carrier of  claim 5 , wherein the drug is an antibody, an antibody fragment, DNA, RNA, siRNA, a protein, a stem cell, or a chemical compound. 
     
     
         7 . A filler composition comprising the hyaluronic acid hydrogel of  claim 1 . 
     
     
         8 . A composition for adhesion barrier, the composition comprising the hyaluronic acid hydrogel of  claim 1 . 
     
     
         9 . A composition for wound dressing, the composition comprising the hyaluronic acid hydrogel of  claim 1 . 
     
     
         10 . A composition for sustained release of an active agent, the composition comprising the hyaluronic acid hydrogel of  claim 1 . 
     
     
         11 . The hyaluronic acid hydrogel of  claim 2 , wherein the hyaluronic acid derivative has a gallol group substitution rate of about 0.1% to 50%. 
     
     
         12 . A scaffold for tissue engineering, the scaffold comprising the hyaluronic acid hydrogel of  claim 2 . 
     
     
         13 . A drug delivery carrier comprising the hyaluronic acid hydrogel of  claim 2 . 
     
     
         14 . The drug delivery carrier of  claim 13 , wherein the drug is an antibody, an antibody fragment, DNA, RNA, siRNA, a protein, a stem cell, or a chemical compound. 
     
     
         15 . A filler composition comprising the hyaluronic acid hydrogel of  claim 2 . 
     
     
         16 . A composition for adhesion barrier, the composition comprising the hyaluronic acid hydrogel of  claim 2 . 
     
     
         17 . A composition for wound dressing, the composition comprising the hyaluronic acid hydrogel of  claim 2 . 
     
     
         18 . A composition for sustained release of an active agent, the composition comprising the hyaluronic acid hydrogel of  claim 2 .