US2022324905A1PendingUtilityA1

Enhanced protein purification through a modified protein a elution

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Assignee: GENENTECH INCPriority: Sep 1, 2009Filed: Dec 21, 2021Published: Oct 13, 2022
Est. expirySep 1, 2029(~3.1 yrs left)· nominal 20-yr term from priority
Inventors:Arick Michael BrownChristopher John DowdAsha Nandini-Radhamohan Keba
C07K 1/22C07K 1/18C12P 21/005C07K 16/46
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Claims

Abstract

The present invention provides methods for purifying a polypeptide comprising a CH2/CH3 region, comprising binding the polypeptide to Protein A and eluting with a pH gradient starting at a low pH.

Claims

exact text as granted — not AI-modified
1 . A method for purifying a polypeptide comprising a C H 2/C H 3 region, comprising binding the polypeptide to Protein A and eluting with a pH gradient starting at or below 5.0. 
     
     
         2 - 71 . (canceled) 
     
     
         72 . The method of  claim 1 , wherein the polypeptide is an antibody directed against an antigen selected from the group consisting of a CD protein, a cell adhesion molecule, and a growth factor. 
     
     
         73 . The method of  claim 1 , wherein the polypeptide is an antibody directed against an antigen selected from the group consisting of CD3, CD25, and CD52.

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