Soybean event dp-305423-1 and compositions and methods for the identification and/or detection thereof

Abstract

Compositions and methods related to transgenic high oleic acid/ALS inhibitor-tolerant soybean plants are provided. Specifically, the present invention provides soybean plants having a DP-305423-1 event which imparts a high oleic acid phenotype and tolerance to at least one ALS-inhibiting herbicide. The soybean plant harboring the DP-305423-1 event comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:8, 9, 14, 15, 20, 21, 83 or 84. The characterization of the genomic insertion site of the DP-305423-1 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean DP-305423-1 events are provided.

What is claimed: 
     
         1 . A sample comprising an amplicon comprising nucleotides 18,652-31,579 of SEQ ID NO:5, nucleotides 12,164-14,494 of SEQ ID NO:6, nucleotides 5,751-7,813 of SEQ ID NO:7, nucleotides 2,899-7,909 of SEQ ID NO:82, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:83, SEQ ID NO:84, SEQ ID NO:85, SEQ ID NO:86, SEQ ID NO:87, or SEQ ID NO:88, or a full complement thereof. 
     
     
         2 . The sample of  claim 1 , wherein the sample further comprises a probe which hybridizes under stringent hybridization conditions with the amplicon. 
     
     
         3 . The sample of  claim 2 , wherein the probe comprises a detectable label. 
     
     
         4 . The sample of  claim 2 , wherein the probe comprises a fluorescent label. 
     
     
         5 . The sample of  claim 1 , wherein the sample further comprises a primer comprising at least 11 contiguous nucleotides or a full complement thereof of (i) nucleotides 18,652-31,579 of SEQ ID NO:5, (ii) nucleotides 12,164-14,494 of SEQ ID NO:6, (iii) nucleotides 5,751-7,813 of SEQ ID NO:7, or (iv) nucleotides 2899-7909 of SEQ ID NO:82. 
     
     
         6 . The sample of  claim 1 , wherein the amplicon comprises SEQ ID NO: 8, 9, 14, 15, 20, 21, 83 or 84, or a complete complement thereof. 
     
     
         7 . The sample of  claim 2 , wherein the amplicon comprises SEQ ID NO. 8 and wherein the probe hybridizes under stringent hybridization conditions with SEQ ID NO. 8. 
     
     
         8 . The sample of  claim 2 , wherein the amplicon comprises SEQ ID NO. 9 and wherein the probe hybridizes under stringent hybridization conditions with SEQ ID NO. 9. 
     
     
         9 . The sample of  claim 2 , wherein the amplicon comprises SEQ ID NO. 14 and wherein the probe hybridizes under stringent hybridization conditions with SEQ ID NO. 14. 
     
     
         10 . The sample of  claim 2 , wherein the amplicon comprises SEQ ID NO. 15 and wherein the probe hybridizes under stringent hybridization conditions with SEQ ID NO. 15. 
     
     
         11 . The sample of  claim 2 , wherein the amplicon comprises SEQ ID NO. 20 and wherein the probe hybridizes under stringent hybridization conditions with SEQ ID NO. 20. 
     
     
         12 . The sample of  claim 2 , wherein the amplicon comprises SEQ ID NO. 21 and wherein the probe hybridizes under stringent hybridization conditions with SEQ ID NO. 21. 
     
     
         13 . The sample of  claim 2 , wherein the amplicon comprises SEQ ID NO. 83 and wherein the probe hybridizes under stringent hybridization conditions with SEQ ID NO. 83. 
     
     
         14 . The sample of  claim 2 , wherein the amplicon comprises SEQ ID NO.84 and wherein the probe hybridizes under stringent hybridization conditions with SEQ ID NO. 84. 
     
     
         15 . The sample of  claim 2 , wherein the probe comprises SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:83, SEQ ID NO:84, SEQ ID NO:85, SEQ ID NO:86, SEQ ID NO:87, or SEQ ID NO:88, or a full complement thereof. 
     
     
         16 . The sample of  claim 1 , wherein the amplicon comprises a detectable label. 
     
     
         17 . The sample of  claim 16 , wherein the detectable label comprises a fluorescent label. 
     
     
         18 . The sample of  claim 6 , wherein the amplicon comprises a detectable label. 
     
     
         19 . The sample of  claim 18 , wherein the detectable label comprises a fluorescent label. 
     
     
         20 . A recombinant DNA construct comprising: a first and second expression cassette, wherein the first expression cassette in operable linkage comprises:
 (a) a soybean KTi3 promoter;   (b) a gm-fad2-1 fragment comprising from position 2114 to 2710 of SEQ ID NO. 1; and   (c) a soybean KTi3 transcriptional terminator; and   
       the second expression cassette comprising in operable linkage:
 (i) a soybean SAMS promoter; 
 (ii) a soybean SAMS 5′ untranslated leader and intron; 
 (iii) a soybean gm-hra encoding DNA molecule comprising from position 1534 to 3504 of SEQ ID NO. 2; and 
 (iv) a soybean als transcriptional terminator.