Tissue array printing

Abstract

Disclosed herein are methods of printing a bio-ink on a substrate. The methods further encompass methods of printing a live tissue and methods of treating tissue defects.

1 . A system comprising:
 a) a controller, and   b) a printhead, wherein the printhead comprises a plurality of rows comprising a plurality of print nozzles, wherein the plurality of print nozzles is configured to be positioned at varying heights.   
     
     
         2 . The system of  claim 1 , wherein at least one print nozzle of the plurality of print nozzles is independently actuated to print an individual droplet. 
     
     
         3 . The system of  claim 1 , wherein the plurality of print nozzles is within 5 cm of a substrate. 
     
     
         4 . The system of  claim 3 , wherein the substrate is a tissue defect. 
     
     
         5 . The system of  claim 1 , wherein a bio-ink is ejected from the plurality of print nozzles. 
     
     
         6 . The system of  claim 1 , wherein the plurality of print nozzles fire simultaneously. 
     
     
         7 . The system of  claim 1 , wherein the plurality of print nozzles fire in a specific sequence. 
     
     
         8 . The system of  claim 1 , wherein the printhead is mounted on a robotic arm for positioning the printhead. 
     
     
         9 . The system of  claim 1 , wherein the spacing between each of the print nozzles of the plurality of print nozzles is between 5 micrometers and 500 micrometers. 
     
     
         10 . The system of  claim 1 , wherein the plurality of print nozzles are mounted in fixed positions relative to one another. 
     
     
         11 . The system of  claim 1 , wherein the plurality of print nozzles are not in the same plane and a pattern of the plurality of print nozzles is customized to the substrate's pattern. 
     
     
         12 . The system of  claim 1 , wherein a first print nozzle is configured to be at an angle compared to the configuration of a second print nozzle. 
     
     
         13 . The system of  claim 12 , wherein the plurality of print nozzles is configured to be a linear array of converging print nozzles. 
     
     
         14 . The system of  claim 12 , wherein the plurality of print nozzles is configured to be a linear array of diverging print nozzles. 
     
     
         15 . The system of  claim 12 , wherein the first print nozzle of the plurality of print nozzles can be configured to be at a first angle relative to the second print nozzle at a first time point and configured to be at a second angle relative to the second print nozzle at a second time point. 
     
     
         16 . A printhead comprising a plurality of rows comprising a plurality of print nozzles, wherein the plurality of print nozzles is configured to be positioned at varying heights. 
     
     
         17 - 27 . (canceled) 
     
     
         28 . The printhead of  claim 16 , wherein the printhead comprises a resolution of at least about 100 dots per inch (dpi). 
     
     
         29 . The printhead of  claim 28 , wherein the printhead further comprises a temperature control apparatus. 
     
     
         30 . The printhead of  claim 16 , wherein each print nozzle of the plurality of print nozzles can move independently from one another. 
     
     
         31 - 33 . (canceled) 
     
     
         34 . The printhead of  claim 16 , wherein a first print nozzle of the plurality of print nozzles can be configured to be at a first angle relative to a second print nozzle at a first time point and configured to be at a second angle relative to the second print nozzle at a second time point. 
     
     
         35 - 46 . (canceled)