USE OF iNOS INHIBITORS TO INCREASE VIRAL YIELD IN CULTURE

Abstract

The use of iNOS inhibitors, including aurintricarboxylic acid, dexamethasone and valproic acid, to increase the yield of a variety of viruses in culture, including recombinant herpesviruses is described.

1 - 8 . (canceled) 
     
     
         9 . A method for producing an HSV-1 d27.1 vector comprising:
 (a) infecting V27 cells with HSV-1 d27.1 vector; and   (b) culturing said infected V27 cells in a cell culture medium comprising valproic acid or dexamethasone in an amount that increases HSV-1 d27.1 viral titer.   
     
     
         10 . (canceled) 
     
     
         11 . The method of  claim 9 , wherein said cell culture medium further comprises serum. 
     
     
         12 . The method of  claim 11 , wherein the serum is fetal bovine serum. 
     
     
         13 - 14 . (canceled) 
     
     
         15 . A method for producing herpes simplex virus (HSV) comprising culturing cells infected with said HSV in a cell culture medium that comprises valproic acid or dexamethasone in an amount that increases yield of the HSV as compared with a cell culture medium that lacks the valproic acid or dexamethasone. 
     
     
         16 . The method of  claim 15 , wherein said HSV is herpes simplex-1 virus (HSV-1). 
     
     
         17 . The method of  claim 16 , wherein said HSV-1 is a wild-type HSV-1. 
     
     
         18 . The method of  claim 16 , wherein said HSV-1 is a recombinant HSV-1. 
     
     
         19 . The method of  claim 15 , wherein the cells are 293 or Vero cells. 
     
     
         20 . The method of  claim 19 , wherein the cells are Vero cells that are V27 cells. 
     
     
         21 . The method of  claim 15 , wherein the cell culture medium further comprises serum. 
     
     
         22 . The method of  claim 21 , wherein the serum is fetal bovine serum.