Customized hybrid bone-implant grafts

Abstract

The present invention provides customized hybrid bone-implant grafts and a method of manufacture thereof.

1 - 59 . (canceled) 
     
     
         60 . A hybrid bone-implant graft comprising:
 a) an implant material; and   b) an engineered bone tissue graft having a scaffold, wherein the graft is composed of cells seeded on the scaffold and cultured to promote attachment of the cells to the implant material.   
     
     
         61 . A kit for generating a hybrid bone-implant graft comprising:
 a) one or more populations of tissue forming cells;   b) one or more scaffolds; and   c) an implant material.   
     
     
         62 . A perfusion bioreactor comprising:
 a) one or more graft chambers;   b) a culture medium reservoir; and   c) a tubing circuit;   
       wherein each of the one or more graft chambers has a size and geometry corresponding to a tissue graft or segment thereof 
     
     
         63 . A method of preparing a hybrid bone-implant graft, the method comprising culturing one or more populations of cells on a scaffold to form a tissue graft, wherein the tissue graft is cultured with an implant material to promote attachment of the cells to the implant material. 
     
     
         64 . The method of  claim 63 , wherein the tissue graft is a bone graft. 
     
     
         65 . The method of  claim 63 , wherein the tissue graft is a cartilage graft. 
     
     
         66 . The method of  claim 63 , wherein the tissue graft is vascularized. 
     
     
         67 . The method of  claim 63 , wherein the tissue graft has a thickness of about one centimeter or less. 
     
     
         68 . The method of  claim 63 , wherein the tissue graft has a thickness of from about 0.3 millimeters to about 10 millimeters. 
     
     
         69 . The method of  claim 63 , wherein the scaffold is generated using computer assisted manufacturing, three-dimensional printing, casting, milling, laser cutting, rapid prototyping, or any combination thereof. 
     
     
         70 . The method of  claim 63 , wherein the cells are derived from induced pluripotent stem cells. 
     
     
         71 . The method of  claim 63 , wherein the cells comprise bone-forming cells and/or cells capable of differentiating into bone-forming cells. 
     
     
         72 . The method of  claim 63 , wherein the cells comprise blood vessel-forming cells and/or cells capable of differentiating into blood vessel-forming cells. 
     
     
         73 . The method of  claim 63 , wherein the cells comprise endothelial progenitor cells. 
     
     
         74 . A method of forming the hybrid bone-implant graft of  claim 63 , the method comprising attaching the tissue graft to the implant material. 
     
     
         75 . The method of  claim 74 , wherein the tissue graft is seeded with cells before attachment to the implant material. 
     
     
         76 . The method of  claim 74 , wherein the tissue graft is seeded with cells after attachment to the implant material. 
     
     
         77 . The method of  claim 74 , wherein the implant material and tissue graft are threadably attached. 
     
     
         78 . The method of  claim 74 , wherein the implant material and tissue graft are manually attached or attached via a mechanized tool. 
     
     
         79 . The method of  claim 74 , wherein the implant material and tissue graft are attached by boring a hole into the tissue graft and inserting the implant material into the hole.