Protective enzymes

Abstract

The present invention relates to methods and compositions that include enzymes and/or binding polypeptides useful for protecting polymers from damage caused by fatty acids from secreted biological fluids such as sebum or sweat.

1 - 34 . (canceled) 
     
     
         35 . A host cell that comprises a heterologous polynucleotide encoding a lipoxygenase, wherein the lipoxygenase comprises an amino acid sequence that is at least 90% identical to SEQ ID NO: 2. 
     
     
         36 . The host cell of  claim 35 , wherein the lipoxygenase comprises SEQ ID NO: 2. 
     
     
         37 . The host cell of  claim 35 , wherein the lipoxygenase has activity at the 10S-carbon of one or more fatty acids. 
     
     
         38 . The host cell of  claim 35 , wherein the lipoxygenase promotes breakdown of one or more fatty acids. 
     
     
         39 . The host cell of  claim 38 , wherein the lipoxygenase is capable of promoting the breakdown of one or more fatty acids in bodily fluid secreted from a human. 
     
     
         40 . The host cell of  claim 39 , wherein the bodily fluid comprises sweat or sebum. 
     
     
         41 . The host cell of  claim 38 , wherein the one or more fatty acids is an oleic acid and/or a linoleic acid. 
     
     
         42 . The host cell of  claim 35 , wherein the host cell is a plant cell, a fungal cell, a yeast cell, a bacterial cell, or an animal cell. 
     
     
         43 . The host cell of  claim 42 , wherein the bacterial cell is an  Escherichia coli  cell. 
     
     
         44 . A method comprising culturing the host cell of  claim 35 . 
     
     
         45 . A host cell that comprises a heterologous polynucleotide encoding a lipoxygenase, wherein the lipoxygenase comprises an amino acid sequence that is at least 90% identical to SEQ ID NO: 3. 
     
     
         46 . The host cell of  claim 45 , wherein the lipoxygenase comprises SEQ ID NO: 3. 
     
     
         47 . The host cell of  claim 45 , wherein the lipoxygenase has activity at the 10S-carbon of one or more fatty acids. 
     
     
         48 . The host cell of  claim 45 , wherein the lipoxygenase promotes breakdown of one or more fatty acids. 
     
     
         49 . The host cell of  claim 48 , wherein the lipoxygenase is capable of promoting the breakdown of one or more fatty acids in bodily fluid secreted from a human. 
     
     
         50 . The host cell of  claim 49 , wherein the bodily fluid comprises sweat or sebum. 
     
     
         51 . The host cell of  claim 48 , wherein the one or more fatty acids is an oleic acid and/or a linoleic acid. 
     
     
         52 . The host cell of  claim 45 , wherein the host cell is a plant cell, a fungal cell, a yeast cell, a bacterial cell, or an animal cell. 
     
     
         53 . The host cell of  claim 52 , wherein the bacterial cell is an Escherichia coli cell. 
     
     
         54 . A method comprising culturing the host cell of  claim 45 .