Integrated device with external light source for probing detecting and analyzing molecules

Abstract

Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits radiation; at least one element for directing the emission radiation in a particular direction; and a light path along which the emission radiation travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the integrated device. Each sensor may detect emission radiation from a sample in a respective sample well. The instrument includes an excitation light source for exciting the sample in each sample well.

1 . An integrated device comprising:
 an array of sample wells arranged on a surface of the integrated device;   a first waveguide configured to optically couple excitation energy to a first plurality of sample wells in the array; and   a second waveguide configured to optically couple excitation energy to a second plurality of sample wells in the array.