Electronic label-free dna and genome sequencing

Abstract

A method of manufacturing a device useable in DNA or genome sequencing comprises disposing pairs of electrodes on a substrate, the electrodes within each pair separated by a nanogap; depositing a resist layer over the electrodes; patterning the resist layer to create an exposed region on each electrode at or near each nanogap; roughening the electrode surface within each exposed region using various methods; and exposing the exposed regions to biomolecules, wherein one biomolecule bridges each nanogap of each electrode pair, with each end of each biomolecule bound to the electrodes at each exposed region.

We claim: 
     
         1 . A method of manufacturing a device for DNA or genome sequencing, said method comprising:
 disposing a pair of electrodes on a substrate, the electrodes separated by a nanogap;   depositing a resist layer over the pair of electrodes;   patterning the resist layer to create an exposed region on each electrode at or near the nanogap;   exposing the electrodes to plasma etching or gold (Au) ion beam implantation to roughen the surface within each exposed region; and   exposing the exposed regions to a biomolecule, wherein the biomolecule has at least first and second ends, with each end comprising a functionalization for bonding to the pair of electrodes,
 wherein the biomolecule bridges the nanogap, with the first and second ends of the biomolecule being bound to the exposed regions.