Anti-icos antibodies

Abstract

Antibodies that bind ICOS (Inducible T cell Co-Stimulator). Therapeutic use of anti-ICOS antibodies for modulating the ratio between regulatory T cells and effector T cells, to stimulate the immune system of patients, including use in treating cancers. Methods of producing anti-ICOS antibodies, including species cross-reactive antibodies, using transgenic knock-out mice.

1 - 113 . (canceled) 
     
     
         114 . An isolated nucleic acid encoding an anti-ICOS antibody heavy chain variable domain comprising the amino acid sequence of SEQ ID NO: 408, a light chain variable domain comprising the amino acid sequence of SEQ ID NO:415, or both. 
     
     
         115 . The nucleic acid of  claim 114 , wherein the nucleic acid encodes a heavy chain sequence comprising the amino acid sequence of SEQ ID NO: 410 or a light chain comprising the amino acid sequence of SEQ ID NO:417. 
     
     
         116 . The nucleic acid of  claim 114 , wherein the heavy chain variable domain is encoded by the nucleic acid sequence of SEQ ID NO: 409 or SEQ ID NO: 521 or the light chain variable domain is encoded by the nucleic acid sequence of SEQ ID NO: 416. 
     
     
         117 . The nucleic acid of  claim 116 , wherein the nucleic acid sequence encoding the heavy chain variable domain comprises the nucleic acid sequence of SEQ ID NO:409 and the nucleic acid sequence encoding the light chain variable domain comprises the nucleic acid sequence of SEQ ID NO: 416. 
     
     
         118 . The nucleic acid of  claim 114 , wherein the nucleic acid encoding the heavy chain variable domain and the nucleic acid encoding the light chain variable domain are located on separate vectors. 
     
     
         119 . The nucleic acid of  claim 114 , wherein the nucleic acid encoding the heavy chain variable domain and the nucleic acid encoding the light chain variable domain are on a single vector. 
     
     
         120 . The nucleic acid of  claim 114 , wherein the nucleic acid encodes the heavy chain variable domain and the light chain variable domain. 
     
     
         121 . The nucleic acid of  claim 116 , wherein the nucleic acid encoding the heavy chain variable domain is on a separate vector from a nucleic acid encoding the light chain variable domain. 
     
     
         122 . The nucleic acid of  claim 116 , wherein the nucleic acid encoding the heavy chain variable domain and a nucleic acid encoding the light chain variable domain are on a single vector. 
     
     
         123 . A vector comprising the nucleic acid of  claim 114 . 
     
     
         124 . A vector comprising the nucleic acid of  claim 116 . 
     
     
         125 . A vector comprising the nucleic acid of  claim 118 . 
     
     
         126 . A vector comprising the nucleic acid of  claim 119 . 
     
     
         127 . An isolated recombinant host cell transformed with the nucleic acid of  claim 114 . 
     
     
         128 . An isolated recombinant host cell transformed with the vector of  claim 123 . 
     
     
         129 . An isolated recombinant host cell transformed with the nucleic acid of  claim 116 . 
     
     
         130 . A method of making a ICOS antibody comprising culturing the host cell of  claim 127  under conditions to produce the antibody and recovering the antibody. 
     
     
         131 . A method of making a ICOS antibody comprising culturing the host cell of  claim 128  under conditions to produce the antibody and recovering the antibody. 
     
     
         132 . A method of making a ICOS antibody comprising culturing the host cell of  claim 129  under conditions to produce the antibody and recovering the antibody.