Anti-dll4/vegf dual variable domain immmunoglobulin and uses thereof

Abstract

Disclosed herein are multivalent and multispecific binding proteins, methods of making the binding proteins, and their uses in the diagnosis, monitoring, inhibition, prevention and/or treatment of cancers, tumors, and/or other angiogenesis-dependent diseases diseases characterized by aberrant DLL4 and/or VEGF expression or activity.

1 . A binding protein comprising SEQ ID NO: 73 and 74. 
     
     
         2 .- 24 . (canceled) 
     
     
         25 . An isolated nucleic acid encoding a binding protein amino acid sequence of  claim 1 . 
     
     
         26 . A vector comprising the isolated nucleic acid of  claim 25 . 
     
     
         27 . The vector according to  claim 26 , wherein the vector is pcDNA, pTT, pTT3, pEFBOS, pBV, pJV, pcDNA3.1 TOPO, pEF6 TOPO, pHybE, pBOS or pBJ. 
     
     
         28 . A host cell comprising the vector of  claim 26 . 
     
     
         29 . The host cell according to  claim 28 , wherein the host cell is a prokaryotic cell. 
     
     
         30 . The host cell according to  claim 28 , wherein the host cell is a eukaryotic cell. 
     
     
         31 . The host cell according to  claim 30 , wherein the eukaryotic cell is a protist cell, animal cell, plant cell, yeast cell, mammalian cell, avian cell, insect cell, or fungal cell. 
     
     
         32 .- 121 . (canceled)