Polypeptides having proteolytic activity and use thereof

Abstract

The present invention relates to isolated polypeptides having proteolytic activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors and host cells comprising the polynucleotides, compositions comprising the polypeptides, and methods of producing and using the polypeptides.

1 . An isolated or purified polypeptide having proteolytic activity, selected from the group consisting of:
 (a)
 i. a polypeptide having at least 96%, at least 97%, at least 98%, at least 99% or at least 100% sequence identity to SEQ ID NO: 21, 
 ii. a polypeptide having at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or at least 100% sequence identity to SEQ ID NO: 3, 
 iii. a polypeptide having at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or at least 100% sequence identity to SEQ ID NO: 6, 
 iv. a polypeptide having at least 81%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or at least 100% sequence identity to SEQ ID NO: 9, 
 v. a polypeptide having at least 60%, at least 65%, at least 70%, at least 75, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or at least 100% sequence identity to SEQ ID NO: 12, or 
 vi. a polypeptide having at least 55%, at least 60%, at least 65%, at least 70%, at least 75, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or at least 100% sequence identity to SEQ ID NO: 18, 
   (b) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO: 19, 1, 4, 7, 10, 13 or 16;   (c) a polypeptide derived from a polypeptide of SEQ ID NO: 21, 3, 6, 9, 12, 15 or 18 by substitution, deletion or addition of one or several amino acids in the polypeptide of SEQ ID NO: 21, 3, 6, 9, 12, 15 or 18; and   (d) a fragment of the polypeptide of (a), (b) or (c) that has proteolytic activity.   
     
     
         2 . The polypeptide of  claim 1 , comprising, consisting essentially of, or consisting of SEQ ID NO: 20 or a mature polypeptide thereof; or SEQ ID NO: 21; comprising, consisting essentially of, or consisting of SEQ ID NO: 2 or a mature polypeptide thereof or SEQ ID NO: 3; comprising, consisting essentially of, or consisting of SEQ ID NO: 5 or a mature polypeptide thereof or SEQ ID NO: 6; comprising, consisting essentially of, or consisting of SEQ ID NO: 8 or a mature polypeptide thereof or SEQ ID NO: 9; comprising, consisting essentially of, or consisting of SEQ ID NO: 11 or a mature polypeptide thereof or SEQ ID NO: 12; comprising, consisting essentially of, or consisting of SEQ ID NO: 14 or a mature polypeptide thereof or SEQ ID NO: 15; or comprising, consisting essentially of, or consisting of SEQ ID NO: 17 or a mature polypeptide thereof or SEQ ID NO: 18. 
     
     
         3 . A granule, which comprises:
 (a) a core comprising the polypeptide of  claim 1  and;   (b) a coating consisting of one or more layer(s) surrounding the core.   
     
     
         4 . A granule, which comprises:
 (a) a core, and   (b) a coating consisting of one or more layer(s) surrounding the core, wherein the coating comprises the polypeptide of  claim 1 .   
     
     
         5 . A composition comprising the polypeptide of  claim 1 . 
     
     
         6 . A composition of  claim 5 , wherein the composition is a cleaning composition comprising at least one cleaning component. 
     
     
         7 . The composition according to  claim 5 , wherein the cleaning component is a surfactant and/or a builder. 
     
     
         8 . A cleaning composition comprising:
 a) polypeptide selected from the group consisting of SEQ ID NO: 21, SEQ ID NO: 3, SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 12, SEQ ID NO: 15 and SEQ ID NO: 18, and polypeptides having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% sequence identity to any of said sequences, wherein the polypeptide has proteolytic activity, and   b) at least one surfactant.   
     
     
         9 . A whole broth formulation or cell culture composition comprising the polypeptide of  claim 1 . 
     
     
         10 . An isolated or purified polynucleotide encoding the polypeptide of  claim 1 . 
     
     
         11 . A nucleic acid construct or expression vector comprising the polynucleotide of  claim 10 , wherein the polynucleotide is operably linked to one or more control sequences that direct production of the polypeptide in an expression host. 
     
     
         12 . A recombinant host cell comprising the polynucleotide of  claim 11  operably linked to one or more control sequences that direct production of the polypeptide. 
     
     
         13 . A method of producing a polypeptide having proteolytic activity, comprising cultivating the recombinant host cell of  claim 12  under conditions conducive for production of the polypeptide, and optionally recovering the polypeptide. 
     
     
         14 . (canceled) 
     
     
         15 . A method of cleaning an item, comprising the steps of:
 a) exposing an item to a wash liquor comprising the composition of  claim 5 ,   b) completing at least one wash cycle, and optionally   c) rinsing the item;   wherein the item is a textile or a hard surface.