Bone repair compositions

Abstract

A composition including, (a) a mineral particle, (b) endothelial cells and mesenchymal cells, and (3) hyaluronic acid, is provided. Moreover, a kit which includes: a syringe, a mineral particle covered with endothelial cells and mesenchymal cells organized in 2 or more cell layers attached to the mineral particle, and hyaluronic acid, is also provided. Last, a method for filling a gap in a bone of a subject in need thereof, including contacting the gap with a composition of: (a) a mineral particle, (b) endothelial cells and mesenchymal cells, and (3) hyaluronic acid is provided.

What is claimed is: 
     
         1 . A composition comprising, (a) a mineral particle, (b) endothelial cells and mesenchymal cells, and (3) hyaluronic acid. 
     
     
         2 . The composition of  claim 1 , further comprising albumin. 
     
     
         3 . The composition of  claim 1 , wherein said endothelial cells and mesenchymal cells are organized in 2 or more cell layers, wherein said in 2 or more cell layers are attached to said mineral particle. 
     
     
         4 . The composition of  claim 1 , wherein said 2 or more cell layers attached to said mineral particle are suspended in hyaluronic acid. 
     
     
         5 . The composition of  claim 1 , comprising adipose tissue derived cells. 
     
     
         6 . The composition of  claim 1 , wherein said mineral particle is bone mineral particle. 
     
     
         7 . The composition of  claim 1 , comprising at least 1×10 3  said endothelial cells and mesenchymal cells per 1 mg of said mineral particle. 
     
     
         8 . The composition of  claim 1 , wherein said mesenchymal cells comprise mesenchymal stem cells. 
     
     
         9 . The composition of  claim 1 , wherein said mineral particle comprises: a calcium phosphate derivative, a calcium sulfate derivative, calcium hydroxyapatite, a silicate mineral hydroxyapatite, beta-3 calcium phosphate, or any combination thereof. 
     
     
         10 . The composition of  claim 1 , wherein said mineral particle has a diameter in the range of 50 microns to 2000 microns. 
     
     
         11 . The composition of  claim 5 , comprising 20% to 60% v/v said endothelial cells and mesenchymal cells, said adipose tissue derived cells, or both. 
     
     
         12 . The composition of  claim 1 , maintained in-vitro for 2 to 16 days. 
     
     
         13 . A kit comprising: a syringe, a mineral particle comprising endothelial cells and mesenchymal cells organized in 2 or more cell layers attached thereto, and hyaluronic acid. 
     
     
         14 . The kit of  claim 13 , further comprising a mixing means. 
     
     
         15 . A method for filling a gap in a bone of a subject in need thereof, comprising the step of contacting said gap with a composition comprising: (a) a mineral particle, (b) endothelial cells and mesenchymal cells, and (3) hyaluronic acid, thereby filling a gap in a bone of a subject in need thereof.