In situ filtration for a biocontainer

Abstract

The disclosure herein relates to a TFF assembly including a baffle comprising a microporous filter within a bioreactor. The baffle may include a collection receptacle to capture a target product or waste materials within the baffle after passing through the microporous filter.

1 . A tangential flow filtration (TFF) assembly comprising:
 a microporous membrane; and   a baffle supporting the microporous membrane.   
     
     
         2 . The tangential flow filtration (TFF) assembly of  claim 1 , wherein the baffle rotates or pivots about a point. 
     
     
         3 . The tangential flow filtration (TFF) assembly of  claim 1 , wherein the baffle is a flat plate. 
     
     
         4 . The tangential flow filtration (TFF) assembly of  claim 1 , wherein the baffle is shaped as at least one shape selected from the group consisting of: a rectangle, a trapezoid, a parallelogram, a circle, an ellipse, a racetrack, a triangle, and a ladder. 
     
     
         5 . The tangential flow filtration (TFF) assembly of  claim 1 , further comprising a collection receptacle sealed to the baffle. 
     
     
         6 . The tangential flow filtration (TFF) assembly of  claim 5 , further comprising an outlet on the collection receptacle. 
     
     
         7 . A bioreactor comprising:
 an inner volume enclosed by at least one side wall;   a tangential flow filtration (TFF) assembly including a baffle supporting a microporous membrane, wherein the filtration assembly is movably attached to the side wall; and   a mixer within the inner volume.   
     
     
         8 . The bioreactor of  claim 7 , further comprising one or more inlets or one or more outlets. 
     
     
         9 . The bioreactor of  claim 7 , wherein the baffle spans the height of the inner volume to disrupt vortex formation. 
     
     
         10 . The bioreactor of  claim 7 , wherein the baffle spans enough of the radial dimension of the inner volume to disrupt vortex formation. 
     
     
         11 . The bioreactor of  claim 7 , wherein the bioreactor is single use and/or disposable. 
     
     
         12 . The bioreactor of  claim 7 , wherein the baffle is a flat plate. 
     
     
         13 . The bioreactor of  claim 7 , wherein the baffle is shaped as at least one shape selected from the group consisting of: a rectangle, a trapezoid, a parallelogram, a circle, an ellipse, a racetrack, a triangle, and a ladder. 
     
     
         14 . The bioreactor of  claim 7 , further comprising more than one tangential flow filtration (TFF) assembly. 
     
     
         15 . The bioreactor of  claim 7 , wherein the bioreactor is a perfusion bioreactor. 
     
     
         16 . The bioreactor of  claim 7 , wherein the bioreactor comprises a flexible material. 
     
     
         17 . The bioreactor of  claim 7 , wherein the bioreactor is collapsible. 
     
     
         18 . The bioreactor of  claim 7 , wherein the tangential flow filtration (TFF) assembly is attached to the side wall. 
     
     
         19 . The bioreactor of  claim 7 , further comprising a collection receptacle sealed to the baffle. 
     
     
         20 . The bioreactor of  claim 19 , further comprising an outlet on the collection receptacle. 
     
     
         21 . The bioreactor of  claim 20 , wherein the collection receptacle does not include an outlet. 
     
     
         22 . The bioreactor of  claim 7 , wherein the bioreactor does not include an external pump. 
     
     
         23 . The bioreactor of  claim 7 , wherein the bioreactor does not include a feed line. 
     
     
         24 . The bioreactor of  claim 7 , wherein the bioreactor is self-contained. 
     
     
         25 . The bioreactor of  claim 7 , further comprising an external pump. 
     
     
         26 - 35 . (canceled)