Method and composition for inducing human pluripotent stem cells

Abstract

The present invention provides a method of treating a disorder using a fibromodulin (FMOD) reprogrammed (FReP) cell. The method comprises administering locally to a human being the FReP cell to a site in need thereof of the human being.

We claim: 
     
         1 . A method of treating a disorder in a human patient, comprising administering to the patient a fibromodulin (FMOD) reprogrammed (FReP) cell, generated by a method comprising:
 treating a human cell with a cell culture medium for a period ranging from a day to a month, and   changing the cell culture medium regularly until the FReP cell forms;   wherein the medium comprises fibromodulin (FMOD),   
       wherein the FReP cell expresses NANOG and does not form teratoma, and 
       wherein the human cell is a fibroblast cell 
     
     
         2 . The method of  claim 1 , wherein the disorder is a neurodegenerative disorder. 
     
     
         3 . The method of  claim 1 , wherein the disorder is a central nervous system (CNS) disease, cardiovascular disease, blood diseases, Crohn's disease, bone disease, muscle disease, or chondrocyte disease. 
     
     
         4 . The method of  claim 1 , wherein the disorder is a retina disease, a trauma and injury to a tissue, a skeletal disorder, an organ disease or an injury to skin, muscle, cartilage, tendon, peripheral nerve, spinal cord, blood vessels, or bone. 
     
     
         5 . The method of  claim 1 , wherein the FMOD has a concentration from about 200 nM to about 800 nM. 
     
     
         6 . The method of  claim 1 , wherein the human cell is a BJ fibroblast or primary adult normal human dermal fibroblast (NHDF). 
     
     
         7 . The method of  claim 1 , wherein the FReP cell is included in a composition 
     
     
         8 . The method of  claim 7 , wherein the composition is a pharmaceutical or cosmetic composition.