Antibody-providing kit, antibody-containing patch, method and device for immunoassay using the same

Abstract

The present disclosure relates to an immunoassay method for performing immunoassay by using a patch that contains antibodies. An immunoassay method according to an aspect of the present disclosure performs diagnosis by detecting a target protein from a sample to be diagnosed by using a patch which includes a mesh structural body forming micro-cavities and is configured to contain a liquid substance in the micro-cavities, and includes placing the sample to be diagnosed in a reaction region, and providing an antibodies that react specifically with a target protein to the reaction region by using a patch that contains the antibodies.

1 . An antibody-containing patch comprising:
 antibodies that react specifically with a target protein; and   a mesh structural body provided in a mesh structure forming micro-cavities in which the antibodies are contained that is configured to come into contact with a reaction region in which the target protein is placed and provide some of the contained antibodies to the reaction region.   
     
     
         2 . The antibody-containing patch of  claim 1 , wherein the antibodies that react specifically with the target protein are primary antibodies which specifically bind to a target antigen. 
     
     
         3 . The antibody-containing patch of  claim 1 , wherein the antibodies that react specifically with the target protein are secondary antibodies which specifically bind to antibodies which have a property of binding specifically to a target antigen. 
     
     
         4 . The antibody-containing patch of  claim 1 , wherein:
 the target protein is an antigen;   the antibodies are a pair of antibodies formed by binding of a primary antibody which specifically binds to the antigen and a secondary antibody which specifically binds to the primary antibody; and   the pair of antibodies react specifically with the antigen.   
     
     
         5 . The antibody-containing patch of  claim 1 , wherein:
 the target protein is plural;   the plurality of target protein include a first target protein and a second target protein; and   the antibodies include a first antibody that reacts specifically with the first target protein and a second antibody that reacts specifically with the second target protein.   
     
     
         6 . A patch cluster comprising a plurality of antibody-containing patches, wherein the antibody-containing patch includes antibodies which react specifically with a target protein and a mesh structural body forming micro-cavities in which the antibodies are contained. 
     
     
         7 . The patch cluster of  claim 6 , wherein the plurality of antibody-containing patches include a first antibody-containing patch and a second antibody-containing patch, and a target protein with which a first antibody contained in the first antibody-containing patch reacts specifically is different from a target protein with which a second antibody contained in the second antibody-containing patch reacts specifically. 
     
     
         8 . An immunoassay method for performing diagnosis by detecting a target protein from a sample to be diagnosed by using a patch which includes a mesh structural body forming micro-cavities and is configured to contain a liquid substance in the micro-cavities, the immunoassay method comprising:
 placing the sample to be diagnosed in a reaction region; and   providing antibodies that react specifically with a target protein to the reaction region by using a patch that contains the antibodies.   
     
     
         9 . The immunoassay method of  claim 8 , further comprising:
 providing a substrate, which produces a product through a chemical reaction catalyzed by an enzyme attached to the antibodies, to the reaction region by using a patch that contains the substrate.   
     
     
         10 . The immunoassay method of  claim 8 , further comprising detecting a specific reaction between the antibodies and the target protein to diagnose a target disease. 
     
     
         11 . The immunoassay method of  claim 10 , wherein the detecting of the specific reaction includes detecting the specific reaction by measuring a change in an electrical characteristic of the patch that occurs due to the specific reaction. 
     
     
         12 . The immunoassay method of  claim 10 , wherein the detecting of the specific reaction is performed by any one of measuring fluorescence that occurs due to a chemical reaction catalyzed by an enzyme attached to the antibodies that bind specifically to the target protein, measuring luminescence that occurs due to the chemical reaction, and measuring color that develops due to the chemical reaction. 
     
     
         13 . The immunoassay method of  claim 8 , wherein the placing of the sample to be diagnosed is performed by any one of a method of fixating the sample to the plate, a method of smearing the sample on the plate, and a method of smearing the sample on the plate and fixating the sample. 
     
     
         14 . The immunoassay method of  claim 8 , wherein the providing of the antibodies to the reaction region by using the patch includes contacting the patch with the reaction region so that the antibodies are movable to the reaction region, and separating the patch from the reaction region,
 wherein, when the patch is separated from the reaction region, antibodies that have not reacted specifically with the target protein among the antibodies are removed from the reaction region.   
     
     
         15 . The immunoassay method of  claim 8 , further comprising absorbing antibodies that have not reacted specifically with the target protein among the provided antibodies from the reaction region by using a washing patch. 
     
     
         16 . The immunoassay method of  claim 8 , wherein the providing of the antibodies to the reaction region by using the patch includes:
 using a first patch that contains a first antibody that reacts specifically with the target protein to provide the first antibody to the reaction region; and   using a second patch that contains a second antibody that reacts specifically with the first antibody to provide the second antibody to the reaction region.   
     
     
         17 . The immunoassay method of  claim 8 , wherein:
 the reaction region is located on a plate;   the immunoassay method further comprises, prior to the placing of the sample to be diagnosed in the reaction region, providing the plate on which bottom antibodies, which are antibodies that react specifically with the target protein, are fixated on the reaction region; and   the placing of the sample to be diagnosed in the reaction region includes placing the sample to be diagnosed in the reaction region on which the bottom antibodies are fixated.   
     
     
         18 . The immunoassay method of  claim 8 , wherein:
 the target protein is plural;   the plurality of target proteins include a first target protein and a second target protein; and   the patch contains a first antibody that reacts specifically with the first target protein and a first antibody the reacts specifically with the second target protein.   
     
     
         19 . The immunoassay method of  claim 8 , wherein:
 the target protein is plural;   the patch that contains the antibody is;   the multiple target proteins may include a first target protein and a second target protein; and   the plurality of patches include a first patch that contains a first antibody that reacts specifically with the first target protein and a second patch that contains a second antibody that reacts specifically with the second target protein.   
     
     
         20 . An immunoassay device for performing diagnosis by detecting a target protein from a sample to be diagnosed by using a patch which includes a mesh structural body forming micro-cavities and is configured to contain a liquid substance in the micro-cavities, the immunoassay device comprising:
 a plate supporter configured to support a plate on which a reaction region is placed and a sample to be diagnosed is placed in the reaction region;   a patch controller configured to use the patch, which is configured to contain antibodies that react specifically with the target protein, and control a position of the patch relative to the reaction region so that the antibodies are provided to the reaction region; and   a reaction detector configured to detect a specific reaction between the antibodies and the target protein to diagnose a target disease.   
     
     
         21 . An immunoassay method for performing diagnosis by detecting a target protein from a sample to be diagnosed by using a patch which includes a mesh structural body forming micro-cavities and is configured to contain a liquid substance in the micro-cavities, the immunoassay method comprising:
 placing the sample to be diagnosed in a reaction region;   using a patch that contains a first antibody that reacts specifically with a first target protein to provide the first antibody to the reaction region; and   using a patch that contains a second antibody that reacts specifically with a second target protein to provide the second antibody to the reaction region.   
     
     
         22 . The immunoassay method of  claim 21 , further comprising, after the delivering of the second antibody, detecting the first target protein and the second target protein. 
     
     
         23 . The immunoassay method of  claim 22 , wherein:
 the detecting of the first target protein includes detecting a first fluorescence that is detected from a fluorescence label attached to the first antibody bound specifically to the first target protein; and   the detecting of the second target protein includes detecting a second fluorescence that is detected from a fluorescence label attached to the second antibody bound specifically to the second target protein.   
     
     
         24 . The immunoassay method of  claim 23 , wherein a wavelength band from which the first fluorescence is detected and a wavelength band from which the second fluorescence is detected are different from each other. 
     
     
         25 . The immunoassay method of  claim 21 , further comprising:
 after the providing of the first antibody to the reaction region, detecting the first target protein; and   after the providing of the second antibody to the reaction region, detecting the second target protein.   
     
     
         26 . The immunoassay method of  claim 25 , wherein:
 the detecting of the first target protein includes detecting a first fluorescence that is detected from a fluorescence label attached to the first antibody bound specifically to the first target protein; and   the detecting of the second target protein includes detecting a second fluorescence that is detected from a fluorescence label attached to the second antibody bound specifically to the second target protein.   
     
     
         27 . The immunoassay method of  claim 26 , wherein:
 a wavelength band from which the first fluorescence is detected overlaps at least a portion of a wavelength band from which the second fluorescence is detected; and   the detecting of the second fluorescence is performed by comparing fluorescence detected from the sample after the second antibody is provided to the reaction region and fluorescence detected from the sample before the second antibody is provided to the reaction region.   
     
     
         28 . An antibody providing kit comprising:
 a medium which contains antibodies that react specifically with a target protein; and   an antibody providing patch which includes a mesh structural body forming micro-cavities and is configured to come into contact with the medium to absorb some of the antibodies contained in the medium and come into contact with a reaction region in which the target protein is placed to provide at least some of the absorbed antibodies to the reaction region.   
     
     
         29 . An immunoassay method for performing diagnosis by detecting a target protein from a sample to be diagnosed by using a patch which includes a mesh structural body forming micro-cavities and is configured to contain a liquid substance in the micro-cavities, the immunoassay method comprising:
 contacting a medium, which contains antibodies that react specifically with the target protein, with the patch; and   contacting the patch with a reaction region in which the target protein is placed,   wherein, when the medium is contacted with the patch, at least some of the antibodies contained in the medium are absorbed into the patch.   
     
     
         30 . The immunoassay method of  claim 29 , wherein, when the patch is contacted with the reaction region, at least some of the antibodies absorbed into the patch are movable to the reaction region. 
     
     
         31 . The immunoassay method of  claim 29 , wherein:
 the contacting of the medium with the patch includes contacting a surface of the medium with the patch; and   the contacting of the patch with the reaction region includes contacting a surface of the patch, which is not in contact with the medium, with the reaction region.