Isolated Novel Nucleic Acid and Protein Molecules From Soy and Methods of Using Those Molecules to Generate Transgenic Plants With Enhanced Agronomic Traits

Abstract

This disclosure provides purified nucleic acids and polypeptides. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.

1 . A recombinant DNA construct comprising a heterologous promoter operably linked to a polynucleotide encoding a protein that has an amino acid sequence having at least 95% identity over at least 95% of the length of SEQ ID NO: 166160. 
     
     
         2 . A mixture comprising plant cells, and an antibody to a protein produced in said cells wherein said protein has an amino acid sequence that has at least 95% identity over at least 95% of the length of SEQ ID NO: 166160. 
     
     
         3 . A recombinant DNA construct comprising a heterologous promoter that is functional in a plant cell and that is operably linked to a polynucleotide that
 encodes a protein having an amino acid sequence having at least 95% identity over at least 95% of the length of SEQ ID NO: 166160;   wherein said construct is stably integrated into a chromosome in a plant cell nucleus.   
     
     
         4 . A transgenic plant cell comprising the recombinant DNA construct of  claim 3  wherein said DNA construct provides for an enhanced trait as compared to control plants; and wherein said enhanced trait is enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein or enhanced seed oil. 
     
     
         5 . The plant cell of  claim 4  further comprising DNA expressing a protein that provides tolerance from exposure to an herbicide. 
     
     
         6 . The plant cell of  claim 5  wherein the agent of said herbicide comprises a glyphosate, dicamba, or glufosinate compound. 
     
     
         7 . A transgenic plant comprising a plurality of plant cells of  claim 4 . 
     
     
         8 . The transgenic plant of  claim 7  which is homozygous for said recombinant DNA. 
     
     
         9 . A transgenic seed comprising a plurality of plant cells of  claim 4 . 
     
     
         10 . The transgenic seed of  claim 9  from a corn, soybean, cotton, canola, alfalfa, wheat, rice, sugarcane, or sugar beet plant. 
     
     
         11 . A transgenic pollen grain comprising a haploid derivative of a plant cell nucleus having a chromosome comprising the recombinant DNA construct of  claim 3 . 
     
     
         12 . A method for manufacturing non-natural, transgenic seed that can be used to produce a crop of transgenic plants with an enhanced trait resulting from expression of a stably-integrated, recombinant DNA construct of  claim 3 , said method comprising:
 (a) screening a population of plants for said enhanced trait and said recombinant DNA, wherein individual plants in said population exhibit said trait at a level less than, essentially the same as or greater than the level that said trait is exhibited in control plants which do not contain said recombinant DNA, wherein said enhanced trait is selected from the group of enhanced traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil;   (b) selecting from said population one or more plants that exhibit said trait at a level greater than the level that said trait is exhibited in control plants, and   (c) collecting seed from selected plant from step b.   
     
     
         13 . The method of  claim 12  wherein said method for manufacturing said transgenic seed further comprises:
 (a) verifying that said recombinant DNA is stably integrated in said selected plants, and 
 (b) analyzing tissue of said selected plant to determine the expression of a protein having the function of a protein having an amino acid sequence as set forth in SEQ ID NO: 166160. 
 
     
     
         14 . The method of  claim 13  wherein said seed is corn, soybean, cotton, canola, alfalfa, wheat, rice, sugarcane, or sugar beet seed. 
     
     
         15 . A method of producing hybrid corn seed comprising:
 (a) acquiring hybrid corn seed from an herbicide tolerant corn plant which also has a stably-integrated, recombinant DNA construct of  claim 3 ;   (b) producing corn plants from said hybrid corn seed, wherein a fraction of the plants produced from said hybrid corn seed is homozygous for said recombinant DNA, a fraction of the plants produced from said hybrid corn seed is hemizygous for said recombinant DNA, and a fraction of the plants produced from said hybrid corn seed has none of said recombinant DNA;   (c) selecting corn plants which are homozygous or hemizygous for said recombinant DNA by treating with an herbicide;   (d) collecting seed from herbicide-treated-surviving corn plants and planting said seed to produce further progeny corn plants;   (e) repeating steps (c) and (d) at least once to produce an inbred corn line; and   (f) crossing said inbred corn line with a second corn line to produce hybrid seed.   
     
     
         16 . (canceled)