Customized cosmetic compositions, and methods of rejuvenating and utilizing cells and/or components thereof

Abstract

Cosmetic formulations and compositions customized and/or personalized for rejuvenation of skin and/or hair are described. The cosmetic formulations are customized and/or personalized to include an extract and/or one or more molecular components in amounts and ratios naturally produced by rejuvenated cells or by cells modified toward rejuvenation. These cells are of skin and/or hair origin that have been reprogrammed, in which the skin and/or hair cells, having been rejuvenated and/or modified from the original cell, are initially obtained from a subject for which a customized cosmetic composition is for, or from a subject representative of a person for which a customized cosmetic composition is for. Reprogramming of the skin and/or hair cells provide rejuvenated cells, embryonic stem cell-like pluripotent cells, and/or transdifferentiated or differentiated young or younger cells that have been transdifferentiated from the rejuvenated or modified cells or differentiated from the embryonic stem cell-like pluripotent cells. Methods of selecting, preparing, and formulating the customized cosmetic products are also described.

What is claimed is: 
     
         1 . A method of preparing a cosmetic formulation customized for a subject, the method comprising:
 a) providing a computer system, the computer system comprising:
 i) information relating to the subject from a user's information handling device, wherein the user's information handling device comprises a display screen; 
 ii) a processor connected to a network for receiving the information; 
 iii) a storage device connected to the processor, wherein the storage device is a nontransitory computer readable medium; 
 iv) a computer program in the storage to access, analyze, acquire, collect, compare, enter, store, transmit and/or update information relating to the subject; 
   b) providing a sample containing cells from the subject or a representative of the subject, and using the information relating to the subject to modify the cells by one or more of:
 i) rejuvenating the cells; 
 ii) reprogramming the cells to a less differentiated state; 
 iii) differentiating the cells; 
 iv) transdifferentiating the cells; 
 in any order and any number of repetitions to produce modified cells; 
   c) obtaining an extract from the modified cells and/or their cell culture medium; and   d) providing the extract to the cosmetic formulation.   
     
     
         2 . The method of  claim 1  further comprising providing access to the subject access to the software program to access, acquire, collect, compare, enter, store, transmit and/or update information related to the subject. 
     
     
         3 . The method of  claim 1 , wherein the software program runs on a computer, phone, tablet, or other personal computing device. 
     
     
         4 . The method of  claim 1 , wherein the information relating to the subject comprises one or more of lifestyle, stress, sun exposure, geographic location, skin type, diet, genetic background, cultural background, skin appearance, skin problems, medical history, travel history, cosmetics use, supplement use, drug use, or exercise. 
     
     
         5 . The method of  claim 1 , wherein the information relating to the subject comprises one or more of their social media account information, geographic location, movement, physiological parameters, heartbeat patterns, breathing patterns, or body temperature patterns. 
     
     
         6 . The method of  claim 1 , wherein the software program saves the information relating to the subject to the storage device. 
     
     
         7 . The method of  claim 1  further comprising adding to or removing from the cosmetic formulation one or more cosmetically acceptable additives selected based on information relating to the subject. 
     
     
         8 . The method of  claim 1  wherein the software program provides suggestions to the user for customizing a cosmetic formulation. 
     
     
         9 . The method of  claim 1 , wherein the software program provides the user with the ability to customize the cosmetic formulation. 
     
     
         10 . The method of  claim 1 , wherein the software program provides the user with the ability to buy cosmetic formulations. 
     
     
         11 . The method of  claim 1 , wherein the software program enables the user to save photographs of the appearance of the subject's skin or hair to the storage device. 
     
     
         12 . The method of  claim 1 , wherein the software program enables the user to take photographs of the subject's food, wherein the photographs are used to estimate the composition of the diet of the subject, and wherein software program, optionally, enables the user to save photographs of the subject's food to the storage device. 
     
     
         13 . The method of  claim 1 , wherein the software program enables the user to take photographs of the subject's skin or hair, wherein photographs are used to evaluate the success of a cosmetic formulation. 
     
     
         14 . The method of  claim 11 , wherein the photographs of skin or hair are processed using an artificial intelligence program to estimate the success of the cosmetic formulation. 
     
     
         15 . The method of  claim 11 , wherein the photographs of skin or hair are evaluated by a human to estimate the success of the cosmetic formulation. 
     
     
         16 . The method of  claim 11 , wherein the photographs of skin or hair are evaluated by presenting the photographs to individuals through a computer network or through the software program. 
     
     
         17 . The method of  claim 1 , wherein the information relating to the subject is used in an iterative feedback loop comprising a plurality of cycles, wherein information relating to the subject is collected in each cycle, and wherein the cosmetic formulation is adjusted according to the information relating to the subject, and the adjusted cosmetic formulation is provided to the subject. 
     
     
         18 . The method of  claim 1  further comprising providing access to the subject access to the software program to access, acquire, collect, compare, enter, store, transmit and update information related to the subject. 
     
     
         19 . The method of  claim 18 , wherein:
 (a) the information relating to the subject is used in an iterative feedback loop comprising a plurality of cycles;   (b) the information relating to the subject is collected at a beginning of each cycle; and/or,   (c) the cosmetic formulation is adjusted according to the information relating to the subject, and the adjusted cosmetic formulation is provided to the subject.   
     
     
         20 . The method of  claim 1 , wherein the software program runs on a computer, phone, tablet, or other personal computing device. 
     
     
         21 . The method of  claim 1 , wherein the software program enables the user to take photographs of the subject's food, and wherein the photographs are used to estimate the composition of the diet of the subject. 
     
     
         22 . The method of  claim 21 , wherein the software program enables the user to save photographs of the subject's food to the storage device. 
     
     
         23 . A method of preparing a cosmetic formulation customized for a subject, the method comprising:
 a) providing a computer system, the computer system comprising:
 i) information relating to the subject from a user's information handling device, wherein the user's information handling device comprises a display screen; 
 ii) a processor connected to a network for receiving the information; 
 iii) a storage device connected to the processor, wherein the storage device is a nontransitory computer readable medium; 
 iv) a computer program in the storage to access, analyze, acquire, collect, compare, enter, store, transmit and/or update information relating to the subject; 
   b) providing a sample containing cells from the subject or a representative of the subject, and using the information relating to the subject to rejuvenate the cells by transfecting the cells with:
 (i) one or more synthetic messenger RNAs (mRNA) mimetic encoding a protein selected from each of:
 (1) a telomerase reverse transcriptase (TERT) or a variant thereof, 
 (2) a human sheltering complex component selected from RAP1, TIN2, TRF1, TRF2, TPP1, or POT1, and 
 (3) a human aging/longevity protein selected from SIRT proteins, JunD, GDF11, or growth factor, and 
 
 (ii) one or more of miRNA 10(a), miRNA 21, miRNA-29, miRNA-146(a), miRNA-155, an anti-inflammatory miRNA, or an inhibitor of a pro-inflammatory miRNA; 
   c) culturing the rejuvenated cells in a culture media to generate conditioned media   d) obtaining a cell-free conditioned media from the conditioned media by collecting the conditioned media from the cultured modified rejuvenated skin cells and removing cells from the conditioned media, wherein the cell-free conditioned media is substantially free of the modified rejuvenated skin cells or intracellular or membrane bound components of the modified skin cells; and   d) formulating the cell-free conditioned media in a cosmetic formulation.   
     
     
         24 . The method of  claim 23 , wherein the rejuvenated cells have increased expression of one or more proteins selected from telomerase reverse transcriptase, RAP1, TIN2, TRF1, TRF2, TPP1, POT1, SIRT, SIRT-1, SIRT-2, SIRT-3, SIRT-4, SIRT-5, SIRT-6, SIRT-7, JunD, GDF11, and growth factor. 
     
     
         25 . The method of  claim 23 , wherein the rejuvenated cells have increased expression of the growth factor selected from human fibroblast growth factor (hFGF), basic hFGF (bhFGF), and human stem cell factor (hSCF). 
     
     
         26 . A computer-implemented method of customizing a cosmetic formulation for a subject, the method comprising:
 (a) creating the subject's information profile comprising collecting data relating to one or more of:
 (i) lifestyle, stress, sun exposure, geographic location, skin type, diet, genetic background, cultural background, skin appearance, skin problems, medical history, travel history, cosmetics use, supplement use, drug use, or exercise; and/or 
 (ii) social media account information, geographic location, movement, physiological parameters, heartbeat patterns, breathing patterns, or body temperature patterns; 
   (b) storing the information in a nontransitory computer readable medium, wherein the nontransitory computer readable medium further comprises information regarding cosmetic formulations comprising one or more components selected from additives, modulating components, colorants, fragrances, UV protectants, preservatives, moisturizers, vanishing agents, cosmetically acceptable penetration enhancer, or growth factors;   (c) analyzing and processing information relating to the subject and information regarding cosmetic formulations;   (d) optimizing the one or more components of the cosmetic formulations based on information relating to the subject by adding to or removing from the cosmetic formulations the one or more components of the cosmetic formulations;   (e) deriving or generating a personalized cosmetic formulation based on information relating to the subject; and   (f) providing the subject with the personalized cosmetic formulation.   
     
     
         27 . The computer-implemented method of  claim 26  further comprising formulating a subject-specific customized cosmetic formulation comprising the computer derived or generated personalized cosmetic formulation and modified rejuvenated cell extracts derived from the subject. 
     
     
         28 . The computer-implemented method of  claim 26 , wherein the nontransitory computer readable medium further comprises information regarding rejuvenated cells, pluripotent cells, iPSCs, differentiated young, or younger cells. 
     
     
         29 . The computer-implemented method of  claim 26  further comprising continually updating the subject's information profile and/or information regarding cosmetic formulations. 
     
     
         30 . The computer-implemented method of  claim 29 , wherein the computer automatically derives or generates a new personalized cosmetic formulation based on updated information relating to the subject and/or cosmetic formulations. 
     
     
         31 . The computer-implemented method of  claim 29 , wherein the computer continuously derives or generates new customized cosmetic formulations and the customized cosmetic formulation are adjusted according to the information and the adjusted cosmetic formulation is provided to the subject. 
     
     
         32 . The computer-implemented method of  claim 29 , wherein:
 (a) the information relating to the subject is used in an iterative feedback loop comprising a plurality of cycles;   (b) the information relating to the subject is collected at a beginning of each cycle; and/or,   (c) the customized cosmetic formulation is adjusted according to the information relating to the subject, and the adjusted cosmetic formulation is provided to the subject.