Medical imaging device and methods of use

Abstract

Embodiments related to medical imaging devices including rigid imaging tips and their methods of use for identifying abnormal tissue within a surgical bed are disclosed.

What is claimed is: 
     
         1 . (canceled) 
     
     
         2 . A method for identifying abnormal tissue, comprising:
 illuminating tissue with excitation light including excitation wavelengths between 620 nm and 640 nm, wherein the tissue is marked with pegulicianine;   and collecting a fluorescence signal from the surgical bed using a photosensitive detector.   
     
     
         3 . The method of  claim 2 , wherein the fluorescence signal includes fluorescence wavelengths greater than 660 nm. 
     
     
         4 . The method of  claim 3 , further comprising comparing the fluorescence signal to a fluorescence signal intensity threshold to identify abnormal tissue. 
     
     
         5 . The method of  claim 3 , further comprising indicating one or more locations of the identified abnormal tissue on a screen. 
     
     
         6 . The method of  claim 2 , wherein an intensity of the excitation light is between or equal to 10 mW/cm 2  and 200 mW/cm 2 . 
     
     
         7 . The method of  claim 2 , wherein the photosensitive detector includes a plurality of pixels. 
     
     
         8 . The method of  claim 7 , wherein a field of view per pixel is between about 5 microns and 100 microns inclusively. 
     
     
         9 . The method of  claim 2 , wherein the excitation wavelengths are less than a wavelength cutoff threshold. 
     
     
         10 . The method of  claim 9 , wherein the fluorescence signal includes fluorescence wavelengths greater than the wavelength cutoff threshold. 
     
     
         11 . The method of  claim 10 , wherein the wavelength cutoff threshold is 660 nm. 
     
     
         12 . The method of  claim 2 , wherein the pegulicianine comprises a compound having the formula: 
       
         
           
           
               
               
           
         
       
       or a pharmaceutically acceptable salt thereof. 
     
     
         13 . A medical imaging device comprising:
 a light source configured to illuminate tissue marked with excitation light including excitation wavelengths between 620 nm and 640 nm, wherein the tissue is marked with pegulicianine;   a photosensitive detector configured to detect a fluorescence signal from the tissue; and   a controller configured to identify abnormal tissue based on the fluorescence signal and a fluorescence signal intensity threshold for pegulicianine.   
     
     
         14 . The medical imaging device of  claim 13 , wherein the fluorescence signal includes fluorescence wavelengths greater than 660 nm. 
     
     
         15 . The medical imaging device of  claim 14 , wherein the controller is configured to compare the fluorescence signal to a fluorescence signal intensity threshold to identify the abnormal tissue. 
     
     
         16 . The medical imaging device of  claim 14 , wherein the controller is configured to indicate one or more locations of the abnormal tissue on a screen. 
     
     
         17 . The medical imaging device of  claim 13 , wherein light source is configured to emit the excitation light with an intensity of between or equal to 10 mW/cm 2  and 200 mW/cm 2 . 
     
     
         18 . The medical imaging device of  claim 13 , wherein the photosensitive detector includes a plurality of pixels. 
     
     
         19 . The medical imaging device of  claim 18 , wherein a field of view per pixel is between about 5 microns and 100 microns inclusively. 
     
     
         20 . The medical imaging device of  claim 13 , further comprising a dichroic mirror disposed between a focal plane of the medical imaging device and the photosensitive detector, wherein the excitation wavelengths are less than a wavelength cutoff threshold. 
     
     
         21 . The medical imaging device of  claim 20 , wherein the fluorescence signal includes fluorescence wavelengths greater than the wavelength cutoff threshold. 
     
     
         22 . The medical imaging device of  claim 21 , wherein the wavelength cutoff threshold is 660 nm. 
     
     
         23 . The medical imaging device of  claim 13 , wherein the pegulicianine comprises a compound having the formula: 
       
         
           
           
               
               
           
         
       
       or a pharmaceutically acceptable salt thereof.