Trispecific antibodies

Abstract

Provided herein are trispecific antibodies that include a) a first monomer, b) a second monomer, and c) a light chain. The first monomer includes a first variant Fc domain and a variable heavy domain, the second monomer includes a second variant Fc domain and a first scFv domain and the light chain includes a variable light domain. The variable heavy domain and the variable light domain form an antigen binding domain. The trispecific antibody further includes a second scFv domain on either the first or second monomers.

1 .- 102 . (canceled) 
     
     
         103 . A prostate membrane specific antigen (PSMA) binding domain composition comprising:
 a) a variable heavy domain having an amino sequence of at least 90% sequence identity to SEQ ID NO:219; and   b) a variable light domain having an amino acid sequence of at least 90% sequence identity to SEQ ID NO:223,   wherein the PSMA binding domain is capable of binding to human PSMA.   
     
     
         104 . The PSMA binding domain of  claim 103 , wherein the variable heavy domain has an amino acid sequence of at least 95% sequence identity to SEQ ID NO:219; and wherein the variable light domain has an amino acid sequence of at least 95% sequence identity to SEQ ID NO:223. 
     
     
         105 . The PSMA binding domain of  claim 103 , wherein the variable heavy domain has an amino acid sequence of at least 99% sequence identity to SEQ ID NO:219; and wherein the variable light domain has an amino acid sequence of at least 99% sequence identity to SEQ ID NO:223. 
     
     
         106 . The PSMA binding domain of  claim 103 , wherein the variable heavy domain has the amino acid of SEQ ID NO:219. 
     
     
         107 . The PSMA binding domain of  claim 104 , wherein the variable light domain has one amino acid substitution as compared to SEQ ID NO:223. 
     
     
         108 . The PSMA binding domain of  claim 105 , wherein the variable light domain has one amino acid substitution as compared to SEQ ID NO:223. 
     
     
         109 . A prostate membrane specific antigen (PSMA) binding domain composition comprising:
 a) a variable heavy domain having an amino sequence of SEQ ID NO:219; and   b) a variable light domain having an amino acid sequence of SEQ ID NO:223.