Removing Polyphenol Contaminants from Feedstock-based Polyphenols

Abstract

A method of producing a mixture of pure feedstock-based native polyphenols from a feedstock. Contaminant polyphenols are first removed from an enzyme solution for converting feedstock to a product to produce a polyphenol reduced enzyme solution. The polyphenol reduced enzyme solution is combined with the feedstock and the feedstock is converted to a product and by-product. Heretofore, there has been no process available to reduce or remove the contaminant phenols introduced to the feedstock by commercial enzyme solutions. This method allows for the removal of contaminant phenols prior to introduction to the processing stream and subsequent harvesting of pure feedstock—based native polyphenols. The pure feedstock-based polyphenols are removed from the product or by-product to produce a pure mixture of feedstock-based polyphenols.

1 . A method of producing a pure mixture of native feedstock-based polyphenols from a feedstock for making alcohols or sugars, comprising the steps of:
 prior to enzymatic processing of the feedstock removing contaminating polyphenols from an enzyme solution for converting feedstock to an alcohol or sugar to produce a polyphenol removed enzyme solution;   combining the polyphenol removed enzyme solution with the feedstock;   enzymatic processing of the feedstock without contaminating phenols being present by converting the feedstock to at least one of alcohol or sugar, wherein contaminating polyphenols are phenols or polyphenols not sourced from the feedstock; and   producing the pure mixture of native feedstock-based polyphenols from the feedstock free of the contaminating polyphenols from the enzyme solution by separating native feedstock-based polyphenols from at least one of the alcohol, the sugar, or a by-product of the conversion of the feedstock to the sugar or the alcohol, wherein a native composition of the feedstock-based polyphenols is preserved in the pure mixture of native feedstock-based polyphenols and the pure mixture of native feedstock-based polyphenols free of a contaminating polyphenols is suitable for consumption.   
     
     
         2 . The method of  claim 1 , wherein the feedstock comprises a grain. 
     
     
         3 . The method of  claim 2 , wherein the grain comprises at least one of wheat, corn, barley, sorghum, oats, rye, soybean or canola. 
     
     
         4 . The method of  claim 1 , wherein the by-product comprises an oil. 
     
     
         5 . The method of  claim 1 , wherein the pure mixture of native feedstock-based polyphenols free of a contaminating polyphenols has beneficial human health effects. 
     
     
         6 . The method of  claim 5 , wherein the beneficial health effect is digestive health. 
     
     
         7 . The method of  claim 1 , wherein the pure mixture of native feedstock-based polyphenols free of a contaminating polyphenols is suitable for use as a food additive. 
     
     
         8 . The method of  claim 7 , wherein the pure mixture of native feedstock-based polyphenols free of a contaminating polyphenols prevents oxidation of food. 
     
     
         9 . The method of  claim 1 , wherein the feedstock comprises at least one of sugarcane, sugarbeets, woody biomass, grasses, algae, beans, fruits, and legumes. 
     
     
         10 . The method of  claim 1 , wherein the feedstock is an edible biomass. 
     
     
         11 . A method of producing a pure mixture of native feedstock-based polyphenols from a feedstock comprising a renewable biological material, comprising the steps of:
 prior to enzymatic processing of the feedstock, removing contaminating polyphenols from an enzyme solution for converting the feedstock to a product to produce a polyphenol removed enzyme solution;   combining the polyphenol removed enzyme solution with the feedstock; an-GI   enzymatic processing of the feedstock without contaminating phenols being present by converting the feedstock to the product, wherein contaminating polyphenols are phenols or polyphenols not sourced from the feedstock; and   producing the pure mixture of native feedstock-based polyphenols free of the contaminating polyphenols from the enzyme solution by separating native feedstock-based polyphenols from the product or a by-product of the conversion of the feedstock to the product, wherein a native composition of the feedstock-based polyphenols is preserved in the pure mixture of native feedstock-based polyphenols and the pure mixture of native feedstock-based polyphenols free of a contaminating polyphenols is suitable for consumption.   
     
     
         12 . The method of  claim 11 , wherein the feedstock comprises a grain. 
     
     
         13 . The method of  claim 12 , wherein the grain comprises at least one of wheat, corn, barley, sorghum, oats, rye, soybean or canola. 
     
     
         14 . The method of  claim 11 , wherein the by-product comprises an oil. 
     
     
         15 . The method of  claim 11 , wherein the pure mixture of native feedstock-based polyphenols free of a contaminating polyphenols has beneficial human health effects. 
     
     
         16 . The method of  claim 15 , wherein the beneficial health effect is digestive health. 
     
     
         17 . The method of  claim 11 , wherein the pure mixture of native feedstock-based polyphenols free of a contaminating polyphenols is suitable for use as a food additive. 
     
     
         18 . The method of  claim 17 , wherein the pure mixture of native feedstock-based polyphenols free of a contaminating polyphenols prevents oxidation of food. 
     
     
         19 . The method of  claim 11 , wherein the feedstock comprises at least one of sugarcane, sugarbeets, woody biomass, grasses, algae, beans, fruits, and legumes. 
     
     
         20 . The method of  claim 11 , wherein the feedstock is an edible biomass. 
     
     
         21 . A method of treating an ailment in a human comprising the human consuming an effective amount of the pure mixture of native feedstock-based polyphenols free of the contaminating polyphenols from the enzyme solution formed according to  claim 1 . 
     
     
         22 . A method of treating an ailment in a human comprising the human consuming an effective amount of the pure mixture of native feedstock-based polyphenols free of the contaminating polyphenols from the enzyme solution formed according to  claim 11 .