Methods for antioxidation, preventing osteoporosis and improving immunity by using magnetized elderberry ferment

Abstract

Methods of using magnetized elderberry ferment for antioxidation, preventing osteoporosis and/or improving immunity include administering to a subject in need thereof a composition including a magnetized elderberry ferment, wherein the magnetized elderberry ferment is obtained by sequentially fermenting the elderberry extract with yeast, Streptococcus thermophilus, and Acetobacter aceti in a fermenter under magnetized environment.

What is claimed is: 
     
         1 . A method for antioxidation, comprising administering to a subject in need thereof a composition comprising a magnetized elderberry ferment, wherein the magnetized elderberry ferment is obtained by sequentially fermenting an elderberry extract with yeast,  Streptococcus thermophilus , and  Acetobacter aceti  in a fermenter under a magnetized environment. 
     
     
         2 . The method according to  claim 1 , wherein the magnetized elderberry ferment is capable of increasing total antioxidant capacity (TAC), a concentration of erythrocyte glutathione S-transferase (GST-RBC) and a concentration of f-Thiols w blood of the subject and decreasing a concentration of malondialdehyde (MDA) at the blood of the subject. 
     
     
         3 . The method according to  claim 1 , wherein the magnetized elderberry ferment has a total polyphenol content of 2337.07 μg/mL. 
     
     
         4 . The method according to  claim 1 , wherein the elderberry extract is obtained by mixing an elderberry raw material with water to obtain a mixture and heating the mixture at 80° C.-100° C. for 0.5-1 hour. 
     
     
         5 . The method according to  claim 1 , wherein the magnetized environment is formed by a plurality of magnets arranged outside a body of the fermenter. 
     
     
         6 . The method according to  claim 5 , wherein the magnets are N35 strong neodymium-iron-boron magnets. 
     
     
         7 . A method for preventing osteoporosis, comprising administering to a subject in need thereof a composition comprising a magnetized elderberry ferment, wherein the magnetized elderberry ferment is obtained by sequentially fermenting an elderberry extract with yeast,  Streptococcus thermophilus , and  Acetobacter aceti  in a fermenter under a magnetized environment. 
     
     
         8 . The method according to  claim 7 , wherein the magnetized elderberry ferment promotes bone differentiation. 
     
     
         9 . The method according to  claim 7 , wherein the magnetized elderberry ferment increases concentrations of osteocalcin and type 1 procollagen amino-terminal-propeptide (P1NP) in blood of the subject to enhance bone formation. 
     
     
         10 . The method according to  claim 7 , wherein the magnetized elderberry ferment increases bone density of the subject, 
     
     
         11 . The method according to  claim 7 , wherein the magnetized elderberry ferment has a total polyphenol content of 2337.07 μg/mL. 
     
     
         12 . The method according to  claim 7 , wherein the elderberry extract is obtained by mixing an elderberry raw material with water to obtain a mixture and heating the mixture at 80° C.-100° C. for 0.5-1 hour. 
     
     
         13 . The method according to  claim 7 , wherein the magnetized environment is formed by a plurality of magnets arranged outside a body o the fermenter 
     
     
         14 . The method according to  claim 13 , wherein the magnets are N35 strong neodymium-iron-boron magnets. 
     
     
         15 . A method for improving immunity, comprising administering to a subject in need thereof a composition comprising a magnetized elderberry ferment, wherein the magnetized elderberry ferment is obtained by sequentially fermenting an elderberry extract with yeast,  Streptococcus thermophilus , and  Acetobacter aceti  in a fermenter under a magnetized environment. 
     
     
         16 . The method according to  claim 15 , wherein the magnetized elderberry ferment improves expression level of interleukin-18 gene in the subject and/or improves phagocytic activity of leukocytes in the subject. 
     
     
         17 . The method according to  claim 15 , wherein the magnetized elderberry ferment has a total polyphenol content of 2337.07 μg/mL. 
     
     
         18 . The method according to  claim 15 , wherein the elderberry extract is obtained by mixing an elderberry raw material with water to obtain a mixture and heating the mixture at 80° C.-100° C. for 0.5-1 hour. 
     
     
         19 . The method according to  claim 15 , wherein the magnetized environment is formed by a plurality of magnets arranged outside a body of the fermenter, 
     
     
         20 . The method according to  claim 19 , wherein the magnets are N 35 . strong neodymium-iron-boron magnets.