Methods and systems for visualizing data quality

Abstract

A computer-implemented method for generating a data visualization for operating a digital polymerase chain reaction (dPCR) system comprises, the method comprising receiving fluorescent emission data comprising a plurality of data points corresponding to fluorescence emission from a plurality of reaction sites of a substrate, the fluorescent emission data indicative of presence or absence of amplification product of a dPCR. The method further comprises displaying a data visualization of the plurality of data points in a spatial representation of the substrate such that each data point is displayed at a relative spatial location of its corresponding reaction site of the plurality of reaction sites of the substrate, a first set of data points being displayed with a first indication based on meeting a first quality value threshold and second set of data points being displayed with a second indication, differing from the first indication, based on a second quality value threshold.

What is claimed is: 
     
         1 . A computer-implemented method for generating a data visualization for operating a digital polymerase chain reaction (dPCR) system, the method comprising:
 receiving fluorescent emission data, obtained by a detector, the fluorescent emission data comprising a plurality of data points corresponding to fluorescence emission from a plurality of reaction sites of a substrate, the fluorescent emission data indicative of presence or absence of amplification product of a dPCR reaction at the plurality of reaction sites;   determining a first set of data points of the plurality of data points is of a first data quality level based on a quality threshold;   determining a second set of data points of the plurality of data points is of a second data quality level based on the quality threshold;   displaying on a user interface a data visualization, wherein:   the data visualization displays the plurality of data points in a spatial representation of the substrate such that each data point is displayed at a relative spatial location of its corresponding reaction site of the plurality of reaction sites of the substrate, the first set of data points being displayed with a first indication and the second set of data points being displayed with a second indication differing from the first indication   adjusting the quality threshold to an adjusted quality threshold; and   changing the data visualization displayed on the user interface based on the adjusted quality threshold.