Methods and agricultural compositions for preventing or controlling plant diseases

Abstract

The disclosure provides a method of treating, preventing, and/or controlling plant diseases by applying the agricultural composition comprising a bacterial isolate belonging to Paenibacillus or Bacillus. Also, the disclosure provides a method of enhancing disease resistance of a plant seed by applying the agricultural composition to the plant seed, and an agricultural composition comprising the bacterial isolate.

1 - 73 . (canceled) 
     
     
         74 . An agricultural composition comprising a bacterial isolate of  Paenibacillus  or  Bacillus , or a mutant thereof, wherein the bacterial isolate is fermented and/or cultured in a culture medium comprising BS3, BS3-M2, BS3-M9, BS3-M10, GB6-M, GB6-M3, GB6-M7, GB6-M8, GB6-M9, GB6-M22, GB6-M23, GB6-M 10, GB6-M31, GB6-M33, GB6-M34, or combination thereof. 
     
     
         75 . The agricultural composition of  claim 74 , wherein the culture medium comprises BS3, BS3-M2, GB6-M3, GB6-M10, GB6-M31, GB6-M33, GB6-M34, or combinations thereof. 
     
     
         76 . The agricultural composition of  claim 74 , wherein the bacterial isolate is fermented by a process that comprises:
 (1) inoculating the bacterial isolate in a seed medium and   (2) expanding the culture with a production medium.   
     
     
         77 . The agricultural composition of  claim 76 , wherein the seed medium comprises LB, TSB, BS3, 13S3-M2, BS3-M9, BS3-M10, GB6-M, GB36-M3, GB6-M7, GB6-M8, GB6-M9, GB6-M22, GB6-M23, GB6-M10, GB6-M31, GB6-M33, GB6-M34, or combination thereof. 
     
     
         78 . The agricultural composition of  claim 76 , wherein the production medium comprises BS3, GB6-M3, GB6-M10, GB6-M31, GB6-M33, GB6-M34, or combination thereof. 
     
     
         79 . The agricultural composition of  claim 74 , wherein the concentration of the bacterial isolate is at least 1.3×10 5  cfu/mL, 1.3×10 6  cfu/mL, 1.3×10 7  cfu/mL, 1.3 ×10 8  cfu/lmL, 1.3×10 9  cfu/mL or 1.3×10 10  cfu/mL. 
     
     
         80 . The agricultural composition of  claim 74 , wherein the concentration of the bacterial isolate is from 1×10 5  cfu/mL to 1×10 10  cfu/mL, from 1×10 6  cfu/mL to 5×10 9  cfu/mL, from 1×10 7  cfu/mL to 1×10 9  cfu/mL, or from 5×10 7  cfu/mL to 5×10 8  cfu/mL. 
     
     
         81 . The agricultural composition of  claim 74 , wherein the agricultural composition comprises one or more of an agriculturally acceptable carrier, a wetting agent, a binding agent, a filler, an organic additive, and a surface-active agent. 
     
     
         82 . The agricultural composition of  claim 74 , wherein the agricultural composition comprises an agent, wherein the agent comprises a preservative, a mineral, a thickening agent, a stabilizing agent, a bioprotector, an adjuvant, or combination thereof. 
     
     
         83 . An agricultural composition comprising a bacterial isolate of  Paenibacillus  or  Bacillus , or a mutant thereof, wherein the bacterial isolate is fermented and/or cultured in a culture medium comprising (i) 5-20 g/L Soy peptone, 2-10 g/L Urea, 0-5 g/L CaCl 2 , 2-10 g/L KH 2 PO 4 , 2-10 g/L K 2 HPO 4 , and 10-30 g/L Sucrose; (ii) 10-30 g/L, low fat soy flour, 1-5 g/L CaCl 2 , 2-10 g/L KH 2 PO 4 , 2-10 g/L K 2 HPO 4 , and 10-30 g/L Sucrose; (iii) 5-30 g/L MALTRIN® (M-250 or M-180), 5-25 g/L Dextrose, 1-10 On yeast extract, 0.1-5 g/L ammonia sulfate, and 0.2-3 g/L CaCO 3 ; (iv) 5-40 g/L MALTRIN® (M-250 or M-180), 5-20 g/L Dextrose, 2-15 g/L yeast extract, 2-15 g/L low fat soy flour, 0.2-1.5 g/L Ammonia sulfate, and 0.5-3 g/L CaCO 3 ; or (v) 5-20 g/L low fat soy flour, 0.5-5 g/L CaCl 2 , 2-10 g/L KH 2 PO 4 , 2-10 g/L K 2 HPO 4 , 10-30 g/L Sucrose, and 0.1-5 g/L ammonia sulfate.