Large-field 3d spectral microscopy

Abstract

Optical device for optical fluorescence microscopy, comprising a spatial modulator (1), a microscope objective (2), a beamsplitter (3) and a camera (4), wherein the beamsplitter (3) is placed between the spatial modulator (1) and the microscope objective (2), wherein the beamsplitter (3) is placed between the camera (4) and the microscope objective (2), and wherein a prism (5) is placed between the beamsplitter (3) and the camera (4).

1 - 10 . (canceled) 
     
     
         11 . A wide-field fluorescence microscope, comprising:
 a dichroic beamsplitter;   a spatial modulator;   a camera;   a first optical channel that uses the spatial modulator to transmit illumination light;   a second optical channel extending between the dichroic beamsplitter and an object plane in a three dimensional object medium able to emit fluorescence light in response to the illumination light, wherein the second optical channel transmits the illumination light and the fluorescence light, and wherein the dichroic beamsplitter is dichroic with respect to the illumination light and fluorescence light;   a third optical channel extending between the dichroic beamsplitter and the camera, wherein the third optical channel transmits the fluorescence light from the dichroic beamsplitter to the camera that detects the fluorescence light,   wherein the spatial modulator limits a field of the wide-field fluorescence microscope,   wherein the wide-field fluorescence microscope further comprises an optical means for conjugating the spatial modulator with the object plane for the illumination light and for conjugating the object plane with the camera for the fluorescence light, and   wherein the third optical channel comprises an optical element that is dispersive to the fluorescence light.   
     
     
         12 . The wide-field fluorescence microscope as claimed in  claim 11 , wherein the optical means comprises a microscope objective placed in the second optical channel. 
     
     
         13 . The wide-field fluorescence microscope as claimed in  claim 11 , wherein the optical means comprises a first microscope objective placed in the first optical channel and a second microscope objective placed in the second optical channel. 
     
     
         14 . The wide-field fluorescence microscope as claimed in  claim 11 , wherein the dispersive optical element is a prism. 
     
     
         15 . The wide-field fluorescence microscope as claimed in  claim 11 , wherein the spatial modulator is a digital micromirror device (DMD). 
     
     
         16 . The wide-field fluorescence microscope as claimed in  claim 11 , wherein the spatial modulator is a spinning disk.