Compositions and methods of use for wound healing

Abstract

Described herein are a solution, composition, and kit of poly (acetyl, arginyl) glucosamine (PAAG), methods of making the solution, and method of treating wounds with the solution, the method comprising administering to a subject an effective amount of a solution comprising PAAG, wherein the PAAG when administered topically contacts the wound, thereby treating the wound.

1 - 96 . (canceled) 
     
     
         97 . A method of treating a dermal wound in a subject, the method comprising topically administering to the wound an aqueous pharmaceutical composition comprising:
 a compound of formula (I)   
       
         
           
           
               
               
           
         
         wherein: 
         n is an integer between 20 and 6000; and 
         each R 1  is independently selected for each occurrence from hydrogen, acetyl, 
       
       
         
           
           
               
               
           
         
         wherein at least 25% of R 1  substituents are H, at least 1% of R 1  substituents are acetyl, and at least 2% of R 1  substituents are 
       
       
         
           
           
               
               
           
         
         wherein the compound of formula (I) is present in the composition at a concentration of about 150 to 250 μg/mL, the molecular weight of the compound of formula (I) is from 20 to 150 kDa, and the compound of formula (I) is arginine-functionalized at between 18% to 30%; and 
         a preservative, 
         thereby treating the wound in the subject. 
       
     
     
         98 . The method of  claim 97 , wherein the subject is a human. 
     
     
         99 . The method of  claim 97 , wherein the subject is in the family Elephantidae, Rhinocerotidae, or tapirs. 
     
     
         100 . The method of  claim 97 , wherein the pharmaceutical composition is an osmotically balanced solution further comprising a non-ionic osmol. 
     
     
         101 . The method of  claim 97 , wherein the pharmaceutical composition is administered in a volume sufficient to wash the wound. 
     
     
         102 . The method of  claim 97 , wherein the wound is caused by a chronic disease. 
     
     
         103 . The method of  claim 97 , wherein the wound is a chronic and non-healing dermal or subdermal wound. 
     
     
         104 . The method of  claim 97 , wherein the wound is infected with bacteria. 
     
     
         105 . The method of  claim 97 , wherein the wound is a chronic wound. 
     
     
         106 . The method of  claim 97 , wherein the wound is an acute wound. 
     
     
         107 . The method of  claim 97 , wherein the wound is a burn, puncture, abrasion, laceration, incision, scrape, or excision. 
     
     
         108 . The method of  claim 97 , wherein the pharmaceutical composition is formed by reconstituting a lyophilized composition comprising the compound of formula (I) in a diluent. 
     
     
         109 . The method of  claim 97 , wherein the pharmaceutical composition is administered 1, 2, or 3 times a day. 
     
     
         110 . The method of  claim 97 , wherein the method reduces the healing time or increases the healing rate of the wound. 
     
     
         111 . The method of  claim 110 , wherein the healing time of the wound is reduced by at least about 10% compared to the healing time of the wound that has not been contacted with the pharmaceutical composition. 
     
     
         112 . The method of  claim 97 , wherein the method improves the healing of the wound, wherein healing of the wound results in inflammation, and wherein the inflammation resulting from the healing of the wound is reduced. 
     
     
         113 . The method of  claim 97 , wherein the method decreases the magnitude or extent of scarring. 
     
     
         114 . The method of  claim 97 , wherein the wound, upon treatment, has a reduced bacterial load. 
     
     
         115 . The method of  claim 97 , wherein the method further comprises a second therapy. 
     
     
         116 . The method of  claim 115 , wherein the second therapy is selected from the group consisting of an antibiotic or antibacterial use, a steroidal or non-steroidal anti-inflammatory drug, debridement, irrigation, negative pressure wound therapy, warming, oxygenation, moist wound healing, removing mechanical stress, and adding cells to secrete or enhance levels of healing factors.