Methods for identifying and modulating co-occurant cellular phenotypes

Abstract

The present invention provides tools and methods for the systematic analysis of genetic interactions between cells. The present invention provides tools and methods for modulating cell phenotypes and compositions, combinatorial probing of cellular circuits, for dissecting cellular circuitry, for delineating molecular pathways, and/or for identifying relevant targets for therapeutics development.

1 .- 12 . (canceled) 
     
     
         13 . A cell functionalizing barcoded tag comprising:
 (a) a polyadorned molecule, wherein the molecule comprises 2 to 5 substituents comprising;   (i) a click-enabled moiety, wherein the click-enabled moiety comprises an azide, tetrazine, tetrazole, or nitrone; and,   (ii) an oligonucleotide barcode comprising a spatial barcode.   
     
     
         14 . The cell functionalizing barcoded tag of  claim 13 , wherein the polyadorned molecule comprises a label. 
     
     
         15 . The cell functionalizing barcoded tag of  claim 14 , wherein the label omprises a fluorophore, a peptide-based tag, biotin, a oligonucleotide, a hapten, affinity reagent, lanthanide heavy metal(s) or combination thereof, or a cyanine-based dye. 
     
     
         16 . The cell functionalizing barcoded tag of  claim 15 , wherein the peptide-based tag comprises FLAG-tag, V5 tag, HA-tag, AviTag, Calmodulin-tag, polyglutamate tag, E-tag, His-tag, Myc-tag, S-tag, SBP-tag, Softag 1, Softag 3, Strep-tag, TC tag, VSV-tag, or Xpress tag. 
     
     
         17 .- 34 . (canceled)