Oligonucleotide Paints

Abstract

Novel methods for making high resolution oligonucleotide paints are provided. Novel, high resolution oligonucleotide paints are also provided.

What is claimed is: 
     
         1 . A method comprising:
 contacting a plurality of oligopaints to a target nucleic acid sequence, wherein contacted oligopaints form a barcode or combinatorial color sequence that enables reading of a high number of target nucleic acid sequences.   
     
     
         2 . The method of  claim 1 , wherein the target nucleic acid sequence comprises a DNA sequence or an RNA sequence. 
     
     
         3 . The method of  claim 1 , wherein the target nucleic acid sequence is an oligonucleotide. 
     
     
         4 . The method of  claim 1 , wherein the target nucleic acid sequence is linked to a protein. 
     
     
         5 . The method of  claim 4 , wherein the protein comprises an antibody. 
     
     
         6 . The method of  claim 1 , wherein the contacted oligonucleotide paints are in contact with a retrievable label. 
     
     
         7 . The method of  claim 1 , wherein the oligonucleotide paint is configured to be identifiable with a retrievable label. 
     
     
         8 . The method of  claim 7 , wherein the retrievable label is configured to bind a moiety selected from the group consisting of a protein, a peptide, a DNA sequence, an RNA sequence, and a carbohydrate. 
     
     
         9 . The method of  claim 7 , wherein the protein, the peptide, the DNA sequence, the RNA sequence, and the carbohydrate are in a cell. 
     
     
         10 . The method of  claim 1 , wherein the high number of target nucleic sequences comprises at least one of 50 or more targets, 100 or more targets, 1000 or more targets, 10,000 or more targets. 
     
     
         11 . The method of  claim 1 , wherein the high number of target nucleic acid sequences comprises a number of targets that encode 1-99% of a genome. 
     
     
         12 . The method of  claim 11 , wherein the genome comprises a human genome. 
     
     
         13 . The method of  claim 12 , wherein detecting occurs in a cell, and the method further comprising karyotyping the cell. 
     
     
         14 . The method of  claim 12 , wherein detecting occurs in a cell, and the method further comprises detecting a chromosomal aberration in a cell. 
     
     
         15 . The method of  claim 1 , wherein the contacting occurs within a cell. 
     
     
         16 . The method of  claim 15 , wherein the cell is a human cell. 
     
     
         17 . The method of  claim 1 , wherein detecting occurs in a biological tissue. 
     
     
         18 . The method of  claim 17 , wherein the biological tissue is human tissue.