Anti-yellow fever virus antibodies, and methods of their generation and use

Abstract

Antibodies and antigen-binding fragments thereof specific to the YFV E protein and with neutralizing potency against YFV are provided. These antibodies and antigen-binding fragments are useful in treating YFV.

1 . An isolated antibody or an antigen-binding fragment thereof that specifically binds to a Yellow Fever Virus (YFV) protein, wherein at least one of the CDRH1, a CDRH2, a CDRH3, a CDRL1, a CDRL2, and CDRL3 amino acid sequence of the antibody or the antigen-binding fragment thereof is at least 70% identical to at least one of the CDRH1, a CDRH2, a CDRH3, a CDRL1, a CDRL2, and/or a CDRL3 amino acid sequences as disclosed in Table 3 of an antibody selected from Antibody Number 1 through Antibody Number 152 as disclosed in Table 3; and wherein said antibody or the antigen-binding fragment thereof also has one or more of the following characteristics:
 a) the antibody or antigen-binding fragment thereof cross-competes with said antibody or antigen-binding fragment thereof for binding to YFV;   b) the antibody or antigen-binding fragment thereof displays neutralization activity toward YFV in vitro;   c) the antibody or antigen-binding fragment thereof displays an in vitro neutralization potency (IC 50 ) of between about 0.5 microgram/milliliter (µg/ml) to about 5 µg/ml; between about 0.05 µg/ml to about 0.5 µg/ml; or less than about 0.05 mg/ml; and   d) the antibody or antigen-binding fragment thereof binds to an envelope protein of YFV.   
     
     
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