Automatic dishwashing detergent composition

Abstract

An automatic dishwashing cleaning composition having a new protease.

What is claimed is: 
     
         1 . An automatic dishwashing cleaning composition comprising a variant of a parent protease wherein the parent protease has at least 70% identity with the amino acid sequence of SEQ ID NO:1 and:
 a) the variant comprises an amino acid substitution at position X160Q and
 at least one amino acid substitution at one position selected from: X039E; X099R; X126A; X127E; and X128G; 
   b) the variant comprises an amino acid substitution at position X179Q; and
 at least one amino acid substitution at one position selected from: X099R; X126A; X127E; and X128G; 
 or 
   c) the variant comprises an amino acid substitution at position X253P; and
 at least one amino acid substitution at one position selected from: X039E X099R; X126A; X127E; and X128G; and 
 at least one amino acid substitution at one position selected from: X074D; X211L; X212S and X256E; or 
   d) the variant has one of the following substitutions:
 X039E-X074D-X099R-X126A-X127E-X128G 
 X039E-X099R-X126A-X127E-X128G-X211L-X242D 
 wherein the positions correspond to those of SEQ ID NO: 1. 
   
     
     
         2 . An automatic dishwashing cleaning composition according to  claim 1  wherein
 a) the variant comprises an amino acid substitution at position G160Q and
 at least one amino acid substitution at one position selected from: S039E; S099R; S126A; D127E; and F128G; or 
 
 b) the variant comprises an amino acid substitution at position R179Q; and
 at least one amino acid substitution at one position selected from: S099R; S126A; D127E; and F128G; or 
 
 c) the variant comprises an amino acid substitution at position N253P; and
 at least one amino acid substitution at one position selected from: S039E; S099R; S126A; D127E; and F128G; and 
 at least one amino acid substitutions at one position selected from: N074D; M211L; N212S and Q256E; or 
 
 d) the variant only has one of the following substitutions:
 S039E-S099R-S126A-D127E-F128G-M211L-N242D. 
 
 
     
     
         3 . An automatic dishwashing cleaning composition according to  claim 1  wherein the variant comprises the following substitutions X039E-X099R-X126A-X127E-X128G in combination with one of the following substitutions:
 X074D-X211L-X253P, 
 X179Q-X211L-X253P, 
 X074D-X253P, 
 X085R-X160Q-X179Q-X211L-X212S-X253P, 
 X179Q-X253P, 
 X160Q-X179Q-X211L-X212S-X253P, 
 X160Q-X179Q-X211L-X253P, 
 X160Q-X179Q-X212S-X253P, 
 X160Q-X179Q-X211L-X212S, 
 X074D-X179Q-X211L-X253P, 
 X160Q-X179Q-X211L, 
 X160Q-X179Q-X253P, 
 X074D-X160Q-X212S-X253P, 
 X074D-X160Q-X211L-X253P, 
 X160Q-X179Q, 
 X160Q-X179Q-X212S, 
 X074D-X160Q-X253P, 
 X074D-X160Q-X179Q-X211L-X212S-X253P, 
 X074D-X085R-X160Q-X179Q-X211L, 
 X074D-X160Q-X211L-X212S-X253P, 
 X074D-X160Q-X179Q-X212S-X253P, 
 X074D-X160Q-X211L-X212S, 
 X074D-X160Q, 
 X074D-X160Q-X179Q-X211L-X253P, 
 X074D-X160Q-X212S, 
 X074D-X160Q-X211L, 
 X074D-X160Q-X179Q-X253P, 
 X074D-X160Q-X179Q-X211L-X212S, 
 X074D-X160Q-X179Q-X212S, 
 X074D-X160Q-X179Q-X211L, 
 X074D-X160Q-X179Q, 
 X179Q-X211L-X212S-X253P 
 
     
     
         4 . An automatic dishwashing cleaning composition according to  claim 1  wherein the variant comprises the following substitutions S039E-S099R-S126A-D127E-F128G in combination with one of the following substitutions
 N074D-M211L-N253P, 
 R179Q-M211L-N253P, 
 N074D-N253P, 
 N085R-G160Q-R179Q-M211L-N212S-N253P, 
 R179Q-N253P, 
 G160Q-R179Q-M211L-N212S-N253P, 
 G160Q-R179Q-M211L-N253P, 
 G160Q-R179Q-N212S-N253P, 
 G160Q-R179Q-M211L-N212S, 
 N074D-R179Q-M211L-N253P, 
 G160Q-R179Q-M211L, 
 G160Q-R179Q-N253P, 
 N074D-G160Q-N212S-N253P, 
 N074D-G160Q-M211L-N253P, 
 G160Q-R179Q, 
 G160Q-R179Q-N212S, 
 N074D-G160Q-N253P, 
 N074D-G160Q-R179Q-M211L-N212S-N253P, 
 N074D-N085R-G160Q-R179Q-M211L, 
 N074D-G160Q-M211L-N212S-N253P, 
 N074D-G160Q-R179Q-N212S-N253P, 
 N074D-G160Q-M211L-N212S, 
 N074D-G160Q, 
 N074D-G160Q-R179Q-M211L-N253P, 
 N074D-G160Q-N212S, 
 N074D-G160Q-M211L, 
 N074D-G160Q-R179Q-N253P, 
 N074D-G160Q-R179Q-M211L-N212S, 
 N074D-G160Q-R179Q-N212S, 
 N074D-G160Q-R179Q-M211L, 
 N074D-G160Q-R179Q, 
 R179Q-M211L-N212S-N253P 
 
     
     
         5 . A composition according to  claim 1  wherein the variant has at least about 90% identity to the protease having the amino acid sequence of SEQ ID NO:1. 
     
     
         6 . A composition according to  claim 1  wherein the composition is phosphate free. 
     
     
         7 . A composition according to  claim 1  wherein the composition comprises from about 10% to about 50% by weight of the composition of an organic complexing agent system. 
     
     
         8 . A composition according to  claim 1  comprising more than about 10% by weight of the composition of bleach. 
     
     
         9 . A composition according to  claim 1  comprising a bleach activator and/or a bleach catalyst. 
     
     
         10 . A composition according to  claim 1  comprising a bleach catalyst wherein the bleach catalyst comprises manganese bleach catalyst. 
     
     
         11 . A composition according to  claim 1  wherein the complexing agent system comprises a complexing agent selected from the group consisting of citric acid, methyl glycine diacetic acid, glutamic-N,N-diacetic acid, iminodisuccinic acid, carboxy methyl inulin, their salts, and mixtures thereof, preferably a salt of methyl glycine diacetic acid. 
     
     
         12 . A composition according to  claim 1  wherein the complexing agent system comprises citric acid and methyl glycine diacetic acid preferably in a weight ratio of from about 0.5:1 to about 2:1. 
     
     
         13 . A composition according to  claim 1  wherein the composition comprises a dispersant polymer, preferably a carboxylated/sulfonated polymer. 
     
     
         14 . A composition according to  claim 1  comprising:
 i) from about 10% to about 50% by weight of the composition of an organic complexing agent system, comprising methyl glycine diacetic acid. 
 ii) a bleaching system comprising at least 10% by weight of the composition of percarbonate and optionally a bleach activator and/or a bleach catalyst; 
 iii) a non-ionic surfactant; 
 iv) a dispersant polymer; and 
 v) an amylase. 
 
     
     
         15 . A method of washing soiled dishware in a dishwasher in soft water comprising the steps of:
 i) providing the soiled dishware;   ii) treating the dishware with a cleaning composition according to  claim 1 .