Peptide for Inducing Regeneration of Tissue and Use Thereof

Abstract

(Objective) An objective of the present invention is to provide therapeutic agents that, in association with stimulation of PDGFRα-positive cells such as bone marrow mesenchymal stem cells, promote their mobilization into blood and accumulation in a damaged tissue, and induce tissue regeneration in a living body.(Means for solution) Multiple peptides were synthesized, and the migration-promoting activity of each peptide was evaluated. As a result, the present inventors successfully identified multiple peptides that have migration-promoting activity on a PDGFRα-positive bone marrow mesenchymal stem cell line (MSC-1). Further, the present inventors confirmed that the identified peptides also have migration-promoting activity on skin fibroblasts, which are PDGFRα-positive cells.

1 . A peptide comprising the amino acid sequence of position 17 to position 25 in the amino acid sequence of SEQ ID NO: 1 and having an activity of stimulating migration of a cell, wherein the peptide consists of 195 amino acids or less. 
     
     
         2 . The peptide of  claim 1 , which is a peptide consisting of 185 amino acids or less. 
     
     
         3 . The peptide of  claim 1 , which is a peptide consisting of 84 amino acids or less. 
     
     
         4 . The peptide of  claim 1 , which is a peptide consisting of 44 amino acids or less. 
     
     
         5 . The peptide of  claim 1 , which comprises the amino acid sequences of position 13 to position 25 in the amino acid sequence of SEQ ID NO: 1. 
     
     
         6 . The peptide of  claim 1 , which comprises the amino acid sequences of position 10 to position 25 in the amino acid sequence of SEQ ID NO: 1. 
     
     
         7 . The peptide of  claim 1 , which comprises the amino acid sequences of position 11 to position 44 in the amino acid sequence of SEQ ID NO: 1. 
     
     
         8 . The peptide of  claim 1 , which comprises the amino acid sequences of position 11 to position 34 in the amino acid sequence of SEQ ID NO: 1. 
     
     
         9 . The peptide of  claim 1 , which comprises the amino acid sequences of position 17 to position 30 in the amino acid sequence of SEQ ID NO: 1. 
     
     
         10 . The peptide of  claim 1 , which comprises the amino acid sequences of position 14 to position 30 in the amino acid sequence of SEQ ID NO: 1. 
     
     
         11 . The peptide of  claim 1 , which comprises the amino acid sequences of position 11 to position 30 in the amino acid sequence of SEQ ID NO: 1. 
     
     
         12 . The peptide of  claim 1 , which is a synthetic peptide. 
     
     
         13 . The peptide of  claim 1 , which is a peptide produced using a cell. 
     
     
         14 . The peptide of  claim 1 , which is a peptide to which a tag is added. 
     
     
         15 . The peptide of  claim 1 , which is a peptide to which a tag-derived peptide fragment is added. 
     
     
         16 . A composition comprising the peptide of  claim 1  and a pharmaceutically acceptable carrier or additive. 
     
     
         17 . The composition of  claim 16 , wherein the pharmaceutically acceptable carrier or additive is selected from the group consisting of surfactant, excipient, colorant, perfume, preservative, stabilizer, buffer, suspending agent, isotonizing agent, binder, disintegrant, lubricant, flow promoter, and flavoring agent. 
     
     
         18 . The composition of  claim 16 , wherein the pharmaceutically acceptable carrier or additive is selected from the group consisting of light anhydrous silicic acid, lactose, crystalline cellulose, mannitol, starch, carmellose calcium, carmellose sodium, hydroxypropylcellulose, hydroxypropylmethylcellulose, polyvinylacetaldiethylamino acetate, polyvinylpyrrolidone, gelatin, medium-chain fatty acid triglyceride, polyoxyethylene hydrogenated castor oil 60, white sugar, carboxymethyl cellulose, corn starch, and inorganic salt. 
     
     
         19 . A DNA encoding the peptide of  claim 1 . 
     
     
         20 . A vector comprising the DNA of  claim 19 . 
     
     
         21 . A transformed cell comprising the DNA of  claim 19 . 
     
     
         22 . A transformed cell comprising the vector of  claim 20 .