Method for production of functional polysaccharide and cosmetic composition

Abstract

Provided are a method of manufacturing a functional polysaccharide, the method including: preparing a cell containing a polysaccharide; crushing the cell to extract polysaccharide to the outside of the cell to obtain an extract; and purifying the extract by removing at least one of crude protein, crude fat, and crude powder from the extract, and a cosmetic composition for skin moisturizing or skin elasticity improvement, the cosmetic composition including the functional polysaccharide as an active ingredient.

1 . A method of producing a functional polysaccharide, the method comprising:
 preparing a cell containing a polysaccharide;   obtaining an extract by crushing the cell to extract the polysaccharide to the outside of the cell; and   purifying the extract by removing at least one of crude protein, crude fat, and crude powder from the extract.   
     
     
         2 . The method of  claim 1 , wherein, in the preparing the cell containing the polysaccharide,  E. coli  mutant TBP38 strain or  Saccharomyces Cerevisiae  CEY1 is cultured to prepare the cell. 
     
     
         3 . The method of  claim 1 , wherein, in the obtaining of an extract by crushing the cell to extract the polysaccharide to the outside of the cell,
 the cell is suspended in a citric acid buffer solution having a pH of 3.5 to 4.5, and then crushed using high pressure treatment to extract the functional polysaccharide to the outside of the cell.   
     
     
         4 . The method of  claim 1 , wherein
 the purifying comprises:   heat-treating the extract at 80° C. to 100° C. for 5 minutes to 60 minutes;   adding citric acid to adjust a pH to be 3.5 to 4.5 and removing a precipitate;   adding a detergent thereto to separate layers and recovering a supernatant through centrifugation;   filtering the recovered supernatant through an ultrafine filter and concentrating the same to obtain a concentrate;   adding, to the concentrate, alcohol in an amount 1 to 5 times the volume of the concentrate to recover precipitate; and   re-suspending the precipitate in distilled water or deionized water, and then re-adding alcohol in an amount 1 to 5 times the volume of precipitate and recovering the precipitate.   
     
     
         5 . The method of  claim 1 , wherein an amount of each of crude protein, crude fat, and crude powder is 0.5% or less. 
     
     
         6 . The method of  claim 1 , wherein the polysaccharide is glycogen, and the functional polysaccharide has a purity of 99% or more based on a solid content. 
     
     
         7 . A functional polysaccharide prepared by the method of  claim 1 , wherein an amount of each of crude protein, crude fat, and crude powder is 0.5% or less, and 99% or more of the functional polysaccharide is glycogen based on solid content. 
     
     
         8 . The functional polysaccharide of  claim 7 , wherein the functional polysaccharide has an antioxidant activity. 
     
     
         9 . The functional polysaccharide of  claim 7 , wherein at a concentration of 1000 μg/ml or less, the functional polysaccharide does not have phototoxicity to BALB/3T3 clone A31 cell line. 
     
     
         10 . A cosmetic composition for skin moisturizing or skin elasticity improvement, the cosmetic composition comprising the functional polysaccharide of  claim 7  as an active ingredient.