Bordetella detection assay

Abstract

Disclosed herein are methods and compositions for detecting Bordetella pertussis and Bordetella parapertussis by detecting the presence of the IS481 and IS1001 genomic insertion sequences, respectively.

1 - 33 . (canceled) 
     
     
         34 . An isolated nucleic acid comprising a nucleotide sequence that is at least 95% identical to at least 20 contiguous nucleotides of SEQ ID NO: 2, or a complement thereof, and wherein said nucleic acid is less than 200 nucleotides in length. 
     
     
         35 . The nucleic acid of  claim 34 , wherein said nucleic acid comprises a nucleotide sequence that is identical to at least 20 contiguous nucleotides of SEQ ID NO: 2, or a complement thereof. 
     
     
         36 . The nucleic acid of  claim 35 , wherein said nucleic acid comprises a nucleotide sequence that is identical to at least 40 contiguous nucleotides of SEQ ID NO: 2, or a complement thereof. 
     
     
         37 . The nucleic acid of  claim 36 , wherein said nucleic acid comprises a nucleotide sequence that is identical to SEQ ID NO: 3. 
     
     
         38 . The nucleic acid of  claim 37 , wherein said nucleic acid consists of a nucleotide sequence that is identical to SEQ ID NO: 3. 
     
     
         39 . An isolated nucleic acid comprising a nucleotide sequence that is at least 95% identical to at least 20 contiguous nucleotides of SEQ ID NO: 8, or a complement thereof, and wherein said nucleic acid is less than 200 nucleotides in length. 
     
     
         40 . The nucleic acid of  claim 39 , wherein said nucleic acid comprises a nucleotide sequence that is identical to at least 20 contiguous nucleotides of SEQ ID NO: 8, or a complement thereof. 
     
     
         41 . The nucleic acid of  claim 40 , wherein said nucleic acid comprises a nucleotide sequence that is identical to at least 40 contiguous nucleotides of SEQ ID NO: 8, or a complement thereof. 
     
     
         42 . The nucleic acid of  claim 41 , wherein said nucleic acid comprises a nucleotide sequence that is identical to SEQ ID NO: 9. 
     
     
         43 . The nucleic acid of  claim 42 , wherein said nucleic acid consists of a nucleotide sequence that is identical to SEQ ID NO: 9. 
     
     
         44 - 57 . (canceled) 
     
     
         58 . The nucleic acid of  claim 34 , wherein the nucleic acid is detectably labelled. 
     
     
         59 . The nucleic acid of  claim 39 , wherein the nucleic acid is detectably labelled.