Compositions, apparatus and methods for determining alkalinity of an analyte solution

Abstract

Compositions, kits and methods of using the kits and compositions to determine the alkalinity of an analyte solution are described. The kit can include a lyophilized titrant.

1 - 45 . (canceled) 
     
     
         46 . A method of determining the alkalinity of an analyte composition, the method comprising:
 a) obtaining a microwell plate comprising at least six (6) microwells each having a sequentially increasing amount of a lyophilized titrant composition, wherein the lyophilized titrant composition is in powdered form and comprises an acid, a pH sensitive dye capable of having a colorimetric response in response to a change in pH of a solution, and an excipient;   b) obtaining an aqueous liquid analyte composition;   c) adding substantially the same volume of the aqueous liquid analyte composition to each of the at least six (6) microwells of the microwell plate to form solutions from the aqueous liquid analyte composition and each of the lyophilized titrate compositions in each of the at least six (6) microwells, wherein the pH of the solution in each microwell is different, and wherein each of the lyophilized titrate compositions are dissolved in each solution; and   d) placing the microwell plate in a spectrophotometer and measuring the absorbance value for each solution in each of the plurality of microwells at a first wavelength and a second wavelength, wherein at least one solution represents the alkalinity of the analyte composition.   
     
     
         47 . The method of  claim 46 , wherein the pH of the solution in each microwell is sequentially lower. 
     
     
         48 . The method of  claim 46 , wherein the pH sensitive dye has an acid form and a base form, wherein the absorbance value of the acid form correlates to the first wavelength and the absorbance value of the base form correlates to the second wavelength. 
     
     
         49 . The method of  claim 48 , wherein the pH sensitive dye is a triphenylmethane dye, bromocresol green, crystal violet, cresol red, thymol blue, 2,4-dintrophenol, bromopheol blue, methyl. 
     
     
         50 . The method of  claim 49 , wherein:
 (i) at least one of the solutions has absorbance values as measured at one or more wavelengths that are not statistically differentiable from the absorbance values of a solution with a pH value below that at which the dye shows a colorimetric response, which may be expressed as a ratio of the absorbance of multiple wavelengths, thereby indicating that said solution has an amount of acid that is greater than the amount required to neutralize the alkalinity of the analyte composition; and   (ii) at least one of the solutions has absorbance values as measured at one or more wavelengths that are statistically differentiable from the absorbance values of a solution with a pH value above at which the dye shows a colorimetric response, which may be expressed as a ratio of the absorbance of multiple wavelengths, thereby indicating that said solution has an amount of acid that is less than the amount required to neutralize the alkalinity of the analyte composition.   
     
     
         51 . The method of  claim 46 , wherein the analyte is an aqueous composition obtained from a subsurface well. 
     
     
         52 . The method of  claim 46 , wherein the determined alkalinity value of the analyte correlates to a hardness of the water. 
     
     
         53 . A kit for determining the alkalinity of an analyte composition, the kit comprising:
 a) a microwell plate comprising at least six (6) microwells each having a sequentially increasing amount of a lyophilized titrant composition, wherein the lyophilized titrant composition is in powdered form and comprises an acid, a pH sensitive dye capable of having a colorimetric response in response to a change in pH of a solution, and an excipient; and   b) a spectrophotometer configured to measure the absorbance value of aqueous solutions comprising the lyophilized titrant composition and an aqueous liquid analyte composition at a first wavelength and a second wavelength.