Bryoid compositions, methods of making and use thereof

Abstract

Embodiments of the present invention feature novel Bryoid compositions, methods of making and methods of treating disease.

What is claimed is: 
     
         1 . A method of making a third Bryoid composition comprising the steps of isolating a third Bryoid composition from a source of Bryoids and purifying the third Bryoid composition in a range from 50% to a crystal forming purity wherein said third Bryoid composition has a molecular weight of approximately 868-870 Amu (Mass+Sodium) and 846-848 Amu (monoisotopic mass). 
     
     
         2 . The method of making the third composition of  claim 1  wherein the third Bryoid has a measured mass plus sodium of 869.5 Amu and a measured monoisotopic mass of 846.6 Amu. 
     
     
         3 . The method of making the third Bryoid composition of  claim 1  comprising the steps of:
 (i) extracting the third Bryoid with organic solvents or with SuperFluids (near-critical and supercritical fluids with or without cosolvents) solvents; 
 (ii) partially purifying the third Bryoid by silica chromatography with organic solvents or SuperFluids chromatography; 
 (iii) performing segmentation chromatography on resulting polymeric resin to improve purity of the third Bryoid; 
 (iv) performing C18 chromatography to further improve the purity of the third Bryoid; and 
 (v) crystalizing the third Bryoid to still further improve the purity of the third Bryoid.