Compositions of bioactive fulvate fractions and uses thereof

Abstract

Compositions with fulvate fractions alone or in combination with growth factors, bioactive fragmented peptides, or combinations thereof are disclosed herein. Also disclosed are methods of using said compositions for the catalyzing cellular regeneration, including the healing, treatment, or prevention of skin disorders. Also disclosed are methods for extracting, isolating, and purifying fulvate fractions for use in the manufacture of said compositions.

1 . (canceled) 
     
     
         2 . A composition comprising an isolated fulvate fraction having an average molecular weight ranging from 80 to 350 Da, as measured by vapor pressure osmometry, and a growth factor, wherein the growth factor is fibroblast growth factor (FGF). 
     
     
         3 . The composition of  claim 2 , wherein the isolated fulvate fraction has an average molecular weight ranging from 300 to 320 Da, as measured by vapor pressure osmometry. 
     
     
         4 . The composition of  claim 2 , wherein the isolated fulvate fraction has an average molecular weight of 309 Da, as measured by vapor pressure osmometry. 
     
     
         5 . The composition of  claim 2 , wherein the composition is formulated as a topical formulation. 
     
     
         6 . The composition of  claim 2 , further comprising one or more bioactive peptide. 
     
     
         7 . The composition of  claim 6 , wherein the one or more bioactive peptide is a tigerinin-based peptide. 
     
     
         8 . A method for improving a wound, or skin or hair damage in a subject in need thereof, comprising:
 selecting a subject in need thereof; and   applying a therapeutically effective amount of a composition comprising a fulvate fraction having an approximate molecular formula of C 12 H 16 O 9  and having an average molecular weight ranging from 80 to 350 Da, as measured by vapor pressure osmometry, and a growth factor, wherein the growth factor is fibroblast growth factor (FGF).   
     
     
         9 . The method of  claim 8 , wherein the fulvate fraction has an average molecular weight ranging from 300 to 320 Da, as measured by vapor pressure osmometry. 
     
     
         10 . The method of  claim 8 , wherein the fulvate fraction has an average molecular weight of 309 Da, as measured by vapor pressure osmometry. 
     
     
         11 . The method of  claim 8 , wherein the composition is a topical composition. 
     
     
         12 . The method of  claim 8 , wherein the composition comprises one or more bioactive peptide. 
     
     
         13 . The method of  claim 8 , wherein the one or more bioactive peptide is a tigerinin-based peptide. 
     
     
         14 . A method for isolating a fulvate fraction from humified organic matter (HOM) comprising the fulvate fraction to be isolated, the method comprising:
 separating solids and sludge from the HOM to obtain a solution;   applying an electric current to the solution to obtain a plurality of fulvic acid fractions;   purifying the plurality of fulvic acid fractions;   applying the plurality of fulvic acid fractions to size exclusion chromatography; and   isolating a fulvate fraction, the fulvate fraction having an approximate molecular formula of C 12 H 16 O 9  and having an average molecular weight ranging from 80 to 350 Da as measured by vapor pressure osmometry.   
     
     
         15 . The method of  claim 14 , wherein the HOM is obtained from leonardite. 
     
     
         16 . The method of  claim 14 , wherein separating solids and sludge from the HOM comprises applying the HOM to a settling column. 
     
     
         17 . The method of  claim 14 , further comprising drying the solution before applying an electric current to obtain a flake. 
     
     
         18 . The method of  claim 17 , further comprising reconstituting the flake as a solution. 
     
     
         19 . The method of  claim 14 , wherein purifying the fulvate fraction comprises applying the fulvate fraction to a column of XAD-8. 
     
     
         20 . The method of  claim 14 , wherein applying the fractionated samples to size exclusions chromatography comprises applying the fractionated samples to Fourier transform ion cyclotron resonance mass spectrometry. 
     
     
         21 . The method of  claim 14 , wherein the isolated fulvate fraction is formulated into a topical composition.