Encapsulation of pancreatic cells derived from human pluripotent stem cells

Abstract

The present invention relates to methods for encapsulating pancreatic progenitors in a biocompatible semi-permeable encapsulating device. The present invention also relates to production of human insulin in a mammal in response to glucose stimulation.

What is claimed is: 
     
         1 . A perforated semi-permeable device comprising
 human pancreatic endocrine cells within a semi-permeable membrane comprising a synthetic material, wherein the synthetic material is polysulfone (PST), nano-fiber mats, polyimide, tetrafluoroethylene/polytetrafluoroethylene (PTFE), expanded polytetrafluoroethylene (ePTFE), polyacrylonitrile, polyethersulfone, acrylic resin, cellulose acetate, cellulose nitrate, polyamide, or hydroxylpropyl methyl cellulose (HPMC);   a cell encapsulation chamber; and   at least one seal that is within the cell encapsulation chamber, wherein the at least one seal within the cell encapsulation chamber does not increase the surface area of the cell encapsulation chamber relative to the absence of the at least one seal.   
     
     
         2 . The device of  claim 1 , wherein the device is implanted into a mammalian host. 
     
     
         3 . The device of  claim 2 , wherein the mammalian host is immuno-suppressed. 
     
     
         4 . The device of  claim 1 , wherein the device comprises more than one cell encapsulation chamber. 
     
     
         5 . The device of  claim 1 , wherein the device is refillable. 
     
     
         6 . The device of  claim 1 , wherein the device is vascularized on the exterior and interior of the device. 
     
     
         7 . The device of  claim 1 , wherein the synthetic material is PTFE. 
     
     
         8 . The device of  claim 1 , wherein the synthetic material is ePTFE. 
     
     
         9 . A perforated semi-permeable device comprising
 a human PDX1-positive pancreatic endoderm cell populations within a semi-permeable membrane consisting of a synthetic material, wherein the synthetic material is polysulfone (PSF), nano-fiber mats, polyimide, tetrafluoroethylene/polytetrafluoroethylene (PTFE), expanded polytetrafluoroethylene (ePTFE), polyacrylonitrile, polyethersulfone, acrylic resin, cellulose acetate, cellulose nitrate, polyamide, or hydroxylpropyl methyl cellulose (HPMC);   a cell encapsulation chamber; and   at least one seal that is within the cell encapsulation chamber, wherein the at least one seal within the cell encapsulation chamber does not increase the surface area of the cell encapsulation chamber relative to the absence of the at least one seal.   
     
     
         10 . The device of  claim 9 , wherein the device is implanted into a mammalian host. 
     
     
         11 . The device of  claim 10 , wherein the mammalian host is immuno-suppressed. 
     
     
         12 . The device of  claim 9 , wherein the device comprises more than one cell encapsulation chamber. 
     
     
         13 . The device of  claim 9 , wherein the device is refillable. 
     
     
         14 . The device of  claim 9 , wherein the device is vascularized on the exterior and interior of the device. 
     
     
         15 . The device of  claim 9 , wherein the synthetic material is PTFE. 
     
     
         16 . The device of  claim 9 , wherein the synthetic material is ePTFE. 
     
     
         17 . A perforated semi-permeable device comprising
 a human pancreatic endocrine cell population within a semi-permeable membrane consisting of expanded polytetrafluoroethylene (ePTFE);   a cell encapsulation chamber; and   at least one seal that is within the cell encapsulation chamber, wherein the at least one seal within the cell encapsulation chamber does not increase the surface area of the cell encapsulation chamber relative to the absence of the at least one seal.   
     
     
         18 . The device of  claim 17 , wherein the device comprises more than one cell encapsulation chamber. 
     
     
         19 . The device of  claim 17 , wherein the device is refillable. 
     
     
         20 . The device of  claim 17 , wherein the device is vascularized on the exterior and interior of the device.