Device for processing tissue

Abstract

Preparation of a product comprising stromal vascular traction cells includes washing human biological material comprising adipose in a container apparatus having an internal filter, which divides an internal containment volume of the container apparatus into a tissue retention volume on one side of the filter and a filtrate volume on an opposite side of the filter, and a mixing device with at least one rotatable mixing member disposed in the tissue retention volume. The washing includes operation of the mixing device to rotate the mixing member through the human biological material within the tissue retention volume, and the washing is followed by digesting washed material within the internal containment volume with added enzyme, centrifuging of the container apparatus to prepare a centrifuged pellet in the filtrate volume, selectively removing material of the pellet and preparing a product with a mixture of stromal vascular fraction cells of removed pellet material and an aqueous suspension liquid.

1 .- 17 . (canceled) 
     
     
         18 . An apparatus for processing biological material, the apparatus comprising:
 a container having an internal containment volume, the internal containment volume including a tissue retention volume and a filtrate volume;   a filter disposed within the internal containment volume with the tissue retention volume on one side of the filter and the filtrate volume on another side of the filter and with the tissue retention volume and with the filtrate volume being in fluid communication through the filter;   an inlet port in fluid communication with the tissue retention volume and configured to access the tissue retention volume for introducing human biological material into the tissue retention volume;   an extraction port located on a bottom portion of the container; and   a shaft extending into the tissue retention volume and including one or more mixing members attached to the shaft.   
     
     
         19 . The apparatus according to claim  1 , wherein the shaft is supported by the lid and extends vertically downward from the lid into the tissue retention volume. 
     
     
         20 . The apparatus according to claim  1 , wherein the filter is suspended from the lid. 
     
     
         21 . The apparatus according to claim  1 , comprising an adipose-containing fat graft composition disposed within an available processing volume in the tissue retention volume. 
     
     
         22 . The apparatus according to claim  1 , wherein the inlet port is located on the lid. 
     
     
         23 . The apparatus according to claim  1 , further comprising a suction port located on the lid and in fluid communication with the filtrate volume. 
     
     
         24 . The apparatus according to claim  6 , further comprising a conduit extending from the suction port. 
     
     
         25 . The apparatus according to claim  1 , wherein the inlet port is located on the lid. 
     
     
         26 . The apparatus according to claim  1 , further comprising an access port in fluid communication with the tissue retention volume. 
     
     
         27 . The apparatus according to claim  1 , wherein the mixing members comprise one or more paddles. 
     
     
         28 . A tissue collection and processing system comprising;
 the apparatus according to claim  1 ;   a vacuum system in fluid communication with the apparatus and capable of applying suction to the filtrate volume of the apparatus.   
     
     
         29 . The system according to claim  11 , further comprising a tissue feed conduit in fluid communication with the inlet port of the apparatus to introduce human biological material comprising adipose into the tissue retention volume through the inlet port. 
     
     
         30 . The system according to claim  11 , further comprising a suction port located on the lid and a suction conduit in fluid communication with the suction port and the vacuum system to suction fluid from the filtrate volume of the apparatus. 
     
     
         31 . The system according to claim  12 , wherein the tissue feed conduit is in fluid communication with a lipoplasty cannula. 
     
     
         32 . The system according to claim  11 , further comprising a container in fluid communication with the suction port.