US2023271893A1PendingUtilityA1

Arthrobacter pascens x-1 for promoting growth of nodules and increasing abundance of probiotic microorganism populations

Assignee: UNIV NANJING FORESTRYPriority: Sep 18, 2020Filed: May 24, 2021Published: Aug 31, 2023
Est. expirySep 18, 2040(~14.2 yrs left)· nominal 20-yr term from priority
C05F 11/08C12R 2001/01C05G 3/60C05F 11/00C12N 1/205Y02P60/21C12R 2001/06C05G 3/80C05G 3/00
47
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Claims

Abstract

An Arthrobacter pascens X-1 for promoting a growth of nodules and increasing an abundance of probiotic microorganism populations is disclosed. After the Arthrobacter pascens X-1 provided by the disclosure is applied, nutrient elements required by plants such as potassium, calcium and magnesium in rock powder are effectively released, rock erosion is accelerated to form soil, simultaneously effective nitrogen fixation of soybeans is promoted, nitrogen required for soybean growth is provided, symbiotic nitrogen fixation capability of rhizobium and host plants is obviously exerted, total weight of root nodules is promoted to be increased by 150.00%, content of hydrolyzed nitrogen is improved to be increased by 11.58%, and symbiotic nitrogen fixation capability is effectively exerted. At the same time, the relative abundance of Bradyrhizobium in the soil is increased from 0.21% to 52.47%.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . A method for promoting a growth of nodules, comprising:
 using an  Arthrobacter pascens  X-1 as a bacterial fertilizer, with a preservation number of CCTCC NO: M2019995.   
     
     
         2 . A method for increasing a relative abundance of beneficial microorganisms, comprising:
 using an  Arthrobacter pascens  X-1 as a bacterial fertilizer, with a preservation number of CCTCC NO: M2019995.   
     
     
         3 . An application of an  Arthrobacter pascens  X-1 in promoting a plant nodule proliferation and improving probiotic microorganisms and nutritional environment, comprising:
 using an  Arthrobacter pascens  X-1 as a bacterial fertilizer, wherein the plants are soybean plants.   
     
     
         4 . The application of  claim 3 , wherein a nodule mass of soybean seedlings treated with the X-1 is significantly increased, promoting at least a 150% increase in the total nodule mass. 
     
     
         5 . The application of  claim 4 , wherein after a treatment with the strain X-1, an abundance of the probiotic microorganism population is significantly increased, with a  Bradyrhizobium  increasing from 0.21% to at least 52.47% and a Proteobacteria increasing from 35.36% to at least 69.08%. 
     
     
         6 . An application of  claim 5 , wherein the soybean seedlings treated with the X-1 have a significant increase in the abundance of the probiotic microorganism populations due to an increase in the mass of the nodule, and a significant promoting effect on the plant root system and the aboveground part;
 a root biomass is at least 1.98 g, with a significant increase of 58.40%, a root area is 378.44 cm 2 , with an increase of at least 128.84%, a root volume is 2.54 cm 3 , with an increase of at least 93.89%;   an average aboveground biomass is 10.90 g, with a significant increase of 40.10%;   an average ground diameter is 6.79 mm, with a significant increase of 34.46%;   an average leaf area is at least 87.55 cm 2 , with an increase of over 26.72% the hydrolysis nitrogen is increased by at least 11.58% and the pH is reduced by 6.88 to 6.77.

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