Method for testing different hormones based on different time periods of pregnancy preparation

Abstract

The present invention discloses a method for testing different hormones based on different time periods of pregnancy preparation, including four method steps: fertility test, ovulation test, ovulation confirmation test and pregnancy test. The present invention provides a set of systematic and perfect pregnancy preparation management plan for pregnancy preparation women, which can provide reasonable and effective test guidance for pregnancy preparation women, helps pregnancy preparation women to determine and analyze various hormone test results, and gives corresponding suggestions based on the test results to better help women complete the pregnancy preparation process. The present invention comprehensively and systematically analyzes and determines various hormone levels of women during pregnancy preparation, with high accuracy and reliable analysis results. This pregnancy preparation management method, combined with a hormone test device, enables pregnancy preparation women to complete various hormone tests at home and analyze the test results without going to the hospital frequently, has little impact on life, and brings convenience to the pregnancy preparation women.

1 . A method for testing different hormones based on different time periods of pregnancy preparation, comprising the following steps:
 1) fertility test: on day 3 of a menstrual period, collecting a subject's urine as a test sample, testing the content of follicle-stimulating hormone in the test sample with a hormone test device, and when the content of follicle-stimulating hormone in the urine is more than 15 mIU/mL, prompting damage of the ovarian function or menopause;   2) ovulation test: on day 10-12 of the menstrual period, collecting the subject's urine as a test sample, testing the content of luteinizing hormone in the test sample with a hormone test device, and if the tested content does not reach 10 mIU/mL or more, testing the content of luteinizing hormone again at the same time the next day until the tested content reaches 10 mIU/mL or more; then testing the content of luteinizing hormone once every 4-6 hours until the tested content reaches 35 mIU/mL or more, is and based on the time period of 24-48 hours after the current time as a predicted first ovulation period, prompting the subject to conceive within the first ovulation period; continuing to test the content of luteinizing hormone in the urine every 4-6 hours after the first ovulation period until the tested content is restored to any value below mIU/mL, then arriving at a second ovulation time, prompting the subject to conceive within the second ovulation period;   3) ovulation confirmation test: on at least three consecutive days within 7-10 days after ovulation, collecting the subject's urine as a test sample, testing the content of pregnanediol-3-glucuronide in the test sample with a hormone test device, and if the contents of pregnanediol-3-glucuronide on any two consecutive days within 7-10 days after ovulation exceed 5 μg/mL, confirming that the subject has successfully ovulated during the ovulation period; and   4) pregnancy test: on day 10-14 after the first ovulation time, testing the content of human chorionic gonadotropin in a urine sample with a hormone test device, and when the content tested on any day of day 10-14 after the first ovulation time reaches or exceeds 10 mIU/mL, determining that the subject is pregnant.   
     
     
         2 . The method for testing different hormones based on different time periods of pregnancy preparation according to  claim 1 , wherein the hormone test device shown comprises a test strip ( 1 ), the test strip comprises test paper ( 4 ), and the test paper is provided with a sample adding zone ( 9 ), a marker zone ( 10 ), and a reaction zone ( 8 ); the marker zone ( 10 ) contains one or more specific binding substances a which are marked by markers and can specifically bind to biochemical substances to be tested, the reaction zone ( 8 ) is provided with test lines ( 11 ) corresponding to the biochemical substances to be tested, and each test line ( 11 ) is at least immobilized with one of a corresponding substance of the same type as the biochemical substance to be tested or a specific binding substance b that can specifically bind to the biochemical substance to be tested. 
     
     
         3 . The method for testing different hormones based on different time periods of pregnancy preparation according to  claim 2 , wherein the reaction zone ( 8 ) is further provided with quality control lines ( 12 ), and each quality control line ( 12 ) is quantitatively immobilized with a specific binding substance c capable of binding and capturing the specific binding substance a. 
     
     
         4 . The method for testing different hormones based on different time periods of pregnancy preparation according to  claim 2 , wherein the test paper is further provided with an absorption zone ( 13 ). 
     
     
         5 . The method for testing different hormones based on different time periods of pregnancy preparation according to  claim 2 , wherein the marker is colloidal gold or a fluorescent marker. 
     
     
         6 . The method for testing different hormones based on different time periods of pregnancy preparation according to  claim 2 , wherein the hormone test device further comprises an instrument body ( 2 ), and the instrument body is provided with a display screen ( 18 ), a circuit module ( 16 ), and a light path detection module ( 17 ) capable of performing light detection on the reaction zone of the test paper ( 4 ). 
     
     
         7 . The method for testing different hormones based on different time periods of pregnancy preparation according to  claim 2 , wherein the biochemical substance to be tested is one of or a combination of luteinizing hormone, pregnanediol-3-glucuronide, follicle-stimulating hormone and human chorionic gonadotropin. 
     
     
         8 . The method for testing different hormones based on different time periods of pregnancy preparation according to  claim 2 , wherein the test strip ( 1 ) is provided with a chip ( 7 ) for storing test item information, and the instrument body ( 2 ) is provided with an identification module for identifying and reading the chip ( 7 ).