Lettuce plants having resistance to nasonovia ribisnigri biotype nr:1

Abstract

Lettuce (Lactuca sativa) plants exhibiting resistance to Nasonovia ribisnigri biotype Nr:1 are provided, together with methods of producing, identifying, or selecting plants or germplasm with a Nasonovia ribisnigri biotype Nr:1 resistance phenotype. Such plants include lettuce plants comprising introgressed genomic regions conferring pest resistance. Compositions, including novel polymorphic markers for detecting plants comprising introgressed loci, are further provided.

What is claimed is: 
     
         1 . An elite  Lactuca sativa  plant comprising at least a first recombinant chromosomal segment from  Lactuca serriola  on chromosome 8, wherein said recombinant chromosomal segment comprises an allele conferring resistance to  Nasonovia ribisnigri  biotype Nr:1 relative to a plant lacking said recombinant chromosomal segment. 
     
     
         2 . The plant of  claim 1 , wherein said first recombinant chromosomal segment comprises a marker locus selected from the group consisting of marker locus M1 (SEQ ID NO: 26), marker locus M2 (SEQ ID NO: 16), marker locus M4 (SEQ ID NO: 46), marker locus M5 (SEQ ID NO: 11), marker locus M7 (SEQ ID NO: 21), marker locus M8 (SEQ ID NO: 41), marker locus M10 (SEQ ID NO: 36), and marker locus M11 (SEQ ID NO: 31) on chromosome 8. 
     
     
         3 . The plant of  claim 1 , wherein said  Nasonovia ribisnigri  biotype Nr:1 resistance allele is located between 106,984,777 bp and 136,545,853 bp on chromosome 8 of the public  Lactuca sativa  reference genome Lsat_Salinas_v7. 
     
     
         4 . A plant part of the plant of  claim 1 , wherein said plant part comprises said first recombinant chromosomal segment. 
     
     
         5 . The plant part of  claim 4 , wherein said plant part is a cell, a seed, a root, a stem, a leaf, a head, a flower, or pollen. 
     
     
         6 . A seed that produces the plant of  claim 1 . 
     
     
         7 . The plant of  claim 1 , wherein the plant is homozygous for said recombinant chromosomal segment. 
     
     
         8 . The plant of  claim 1 , wherein said plant further comprises a second recombinant chromosomal segment on chromosome 4, wherein said second recombinant chromosomal segment comprises an allele conferring further improved resistance to  Nasonovia ribisnigri  biotype Nr:1 relative to a plant lacking said second recombinant chromosomal segment. 
     
     
         9 . The plant of  claim 8 , wherein second recombinant chromosomal segment comprises a marker selected from the group consisting of marker locus M13 (SEQ ID NO: 61), marker locus M14 (SEQ ID NO: 66), marker locus M15 (SEQ ID NO: 67), marker locus M16 (SEQ ID NO: 68), marker locus M17 (SEQ ID NO: 69), marker locus M18 (SEQ ID NO: 70), marker locus M19 (SEQ ID NO: 75), marker locus M20 (SEQ ID NO: 76), marker locus M21 (SEQ ID NO: 81), marker locus M22 (SEQ ID NO: 86), and marker locus M23 (SEQ ID NO: 91) on chromosome 4. 
     
     
         10 . A plant part of the plant of  claim 8 , wherein said plant part comprises said first and said second recombinant chromosomal segments. 
     
     
         11 . The plant part of  claim 11 , wherein said plant part is a cell, a seed, a root, a stem, a leaf, a head, a flower, or pollen. 
     
     
         12 . A seed that produces the plant of  claim 8 . 
     
     
         13 . The  Lactuca sativa  plant of  claim 8 , wherein a representative sample of seed comprising said first and said second recombinant chromosomal segments has been deposited under ATCC Accession No. PTA-126067. 
     
     
         14 . A method for producing an elite  Lactuca sativa  plant with improved resistance to  Nasonovia ribisnigri  biotype Nr:1 comprising introgressing into said plant a  Nasonovia ribisnigri  biotype Nr:1 resistance allele within a recombinant chromosomal segment flanked in the genome of said plant by marker locus M5 (SEQ ID NO: 11) and marker locus M4 (SEQ ID NO: 46) on chromosome 8, wherein said introgressed  Nasonovia ribisnigri  biotype Nr:1 resistance allele confers to said plant resistance to  Nasonovia ribisnigri  biotype Nr:1 relative to a plant lacking said allele. 
     
     
         15 . The method of  claim 14 , wherein said introgressing comprises:
 a) crossing a plant comprising said recombinant chromosomal segment with itself or with a second  Lactuca sativa  plant of a different genotype to produce one or more progeny plants; and   b) selecting a progeny plant comprising said recombinant chromosomal segment.   
     
     
         16 . The method of  claim 15 , wherein selecting a progeny plant comprises detecting nucleic acids comprising marker locus M1 (SEQ ID NO: 26), marker locus M2 (SEQ ID NO: 16), marker locus M4 (SEQ ID NO: 46), marker locus M5 (SEQ ID NO: 11), marker locus M7 (SEQ ID NO: 21), marker locus M8 (SEQ ID NO: 41), marker locus M10 (SEQ ID NO: 36), or marker locus M11 (SEQ ID NO: 31). 
     
     
         17 . The method of  claim 15 , wherein the progeny plant is an F 2 -F 6  progeny plant. 
     
     
         18 . The method of  claim 14 , wherein said introgressing comprises backcrossing, marker-assisted selection or assaying for said resistance to  Nasonovia ribisnigr i biotype Nr:1. 
     
     
         19 . The method of  claim 18 , wherein said backcrossing comprises from 2-7 generations of backcrosses. 
     
     
         20 . A  Lactuca sativa  plant obtainable by the method of  claim 14 . 
     
     
         21 . The method of  claim 14 , wherein said plant further comprises a second introgressed  Nasonovia ribisnigri  biotype Nr:1 resistance allele within a recombinant chromosomal segment flanked in the genome of said plant by marker locus M5 (SEQ ID NO: 11) and marker locus M4 (SEQ ID NO: 46) on chromosome 8 or by marker locus M13 (SEQ ID NO: 61) and marker locus M23 (SEQ ID NO: 91) on chromosome 4. 
     
     
         22 . A method of selecting a  Lactuca sativa  plant exhibiting resistance to  Nasonovia ribisnigri  biotype Nr:1, comprising:
 a) crossing the  Lactuca sativa  plant of  claim 1  with itself or with a second  Lactuca sativa  plant of a different genotype to produce one or more progeny plants; and 
 b) selecting a progeny plant comprising said  Nasonovia ribisnigri  biotype Nr:1 resistance allele. 
 
     
     
         23 . The method of  claim 22 , wherein selecting said progeny plant detecting a marker locus genetically linked to said  Nasonovia ribisnigri  biotype Nr:1 resistance allele. 
     
     
         24 . The method of  claim 23 , wherein selecting said progeny plant comprises detecting a marker locus within or genetically linked to a chromosomal segment flanked in the genome of said plant by marker locus M5 (SEQ ID NO: 11) and marker locus M4 (SEQ ID NO: 46) on chromosome 8. 
     
     
         25 . The method of  claim 23 , wherein selecting a progeny comprises detecting nucleic acids comprising marker locus M1 (SEQ ID NO: 26), marker locus M2 (SEQ ID NO: 16), marker locus M4 (SEQ ID NO: 46), marker locus M5 (SEQ ID NO: 11), marker locus M7 (SEQ ID NO: 21), marker locus M8 (SEQ ID NO: 41), marker locus M10 (SEQ ID NO: 36), or marker locus M11 (SEQ ID NO: 31). 
     
     
         26 . The method of  claim 22 , wherein said progeny plant is an F 2 —F 6  progeny plant. 
     
     
         27 . The method of  claim 22 , wherein producing said progeny plant comprises backcrossing.