Multi-sequence capture system

Abstract

Branching phosphoramidite monomers and molecules having comb-like structures are disclosed and described. A branching phosphoramidite monomer having the structure is provided wherein R4 and R5 are independently —(O—CH 2 —CH 2 —) n where n is 1-5 or —O—(CH 2 —) n where n is 1-10, and R1, R2, and R3 are each one of dimethoxytrityl (DMT)-O—, levulinyl (Lev)-O—, and a phosphoramidite.

What is claimed is: 
     
         1 . A comb-like branching molecule having the structure 
       
         
           
           
               
               
           
         
         wherein R1 is —((CH 2 —CH 2 —) n )—R5 where n is 1-5, —((CH 2 —) n )—R5 where n is 1-10, a phosphoramidite, or a combination thereof; 
         R2 is —(CH 2 —CH 2 —) n  where n is 1-5 or —(CH 2 —) n  where n is 1-10; 
         R3 is a spacer; 
         R4 is an oligonucleotide sequence; and 
         R5 is a solid substrate, a reporter molecule, an oligonucleotide, or a combination thereof. 
       
     
     
         2 . The comb-like branching molecule of  claim 1 , wherein the spacer (R3) is —(CH 2 —CH 2 ) n  where n is 1-5, —(CH 2 —) n  where n is 1-10, a phosphoramidite, an oligonucleotide, or a combination thereof. 
     
     
         3 . The comb-like branching molecule of  claim 1 , wherein the spacer includes a cleavable linker. 
     
     
         4 . The comb-like branching molecule of  claim 1 , wherein the oligonucleotide sequence comprises a barcode sequence. 
     
     
         5 . The comb-like branching molecule of  claim 1 , wherein R5 is a solid substrate.