US2023305018A1PendingUtilityA1

Peptide capture and characterization

73
Assignee: LC SCIENCES LLCPriority: Jun 8, 2011Filed: Apr 28, 2022Published: Sep 28, 2023
Est. expiryJun 8, 2031(~4.9 yrs left)· nominal 20-yr term from priority
Inventors:Xiaolian GaoXiaochuan Zhou
G01N 33/575G01N 33/6842B01L 3/5027G01N 33/574B01L 2300/0816B01L 2300/0819
73
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Claims

Abstract

The invention relates to the measurement and profiling of proteins including phosphoproteins from cells in particular the measurement and profiling of phosphoproteins involved in cancer.

Claims

exact text as granted — not AI-modified
1 - 14 . (canceled) 
     
     
         15 . A method for detecting phosphoproteins from cells comprising:
 a) making a microarray containing peptides that bind phosphoproteins;   b) lysing cells and collect all proteins from the cells;   c) binding the phosphoproteins to peptides on the microarray; and   d) detecting the phosphoproteins.   
     
     
         16 . The method of claim  1  wherein the microarray contains peptides that were synthesized in situ. 
     
     
         17 . The method of claim  2  wherein the detection is accomplished using Grb2 as a reporter.

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