Inht26 transgenic soybean

Abstract

Transgenic INHT26 soybean plants comprising modifications of the DAS44406-6 soybean locus which provide for facile excision of the modified DAS44406-6 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

What is claimed is: 
     
         1 . A transgenic soybean plant cell comprising an INHT26 transgenic locus comprising an originator guide RNA recognition site (OgRRS) in a first DNA junction polynucleotide of a DAS44406-6 transgenic locus and a cognate guide RNA recognition site (CgRRS) in a second DNA junction polynucleotide of the DAS44406-6 transgenic locus. 
     
     
         2 . A transgenic soybean plant cell comprising an INHT26 transgenic locus comprising an insertion and/or substitution of DNA in a DNA junction polynucleotide of a DAS44406-6 transgenic locus with DNA comprising a cognate guide RNA recognition site (CgRRS). 
     
     
         3 . The transgenic soybean plant cell of  claim 1  or  2 , wherein said CgRRS comprises the DNA molecule set forth in SEQ ID NO: 17 or 16; and/or wherein said DAS44406-6 transgenic locus is set forth in SEQ ID NO:1, is present in seed deposited at the ATCC under accession No. PTA-11336, is present in progeny thereof, is present in allelic variants thereof, or is present in other variants thereof. 
     
     
         4 . The transgenic soybean plant cell of  claim 1  or  2 , wherein said INHT26 transgenic locus comprises the DNA molecule set forth in SEQ ID NO: 14. 
     
     
         5 . A transgenic soybean plant part comprising the soybean plant cell of any one of  claim 1  or  2 , wherein said soybean plant part is optionally a seed. 
     
     
         6 . A transgenic soybean plant comprising the soybean plant cell of  claim 1  or  2 . 
     
     
         7 . A method for obtaining a bulked population of inbred seed comprising selfing the transgenic soybean plant of  claim 5  and harvesting seed comprising the INHT26 transgenic locus from the selfed soybean plant. 
     
     
         8 . A method of obtaining hybrid soybean seed comprising crossing the transgenic soybean plant of  claim 5  to a second soybean plant which is genetically distinct from the first soybean plant and harvesting seed comprising the INHT26 transgenic locus from the cross. 
     
     
         9 . A DNA molecule comprising SEQ ID NO: 17, 14, or 16. 
     
     
         10 . A processed transgenic soybean plant product comprising the DNA molecule of  claim 9 . 
     
     
         11 . A biological sample containing the DNA molecule of  claim 9 . 
     
     
         12 . A nucleic acid molecule adapted for detection of genomic DNA comprising the DNA molecule of  claim 9 , wherein said nucleic acid molecule optionally comprises a detectable label. 
     
     
         13 . A method of detecting a soybean plant cell comprising the INHT26 transgenic locus of  claim 1  or  2 , comprising the step of detecting DNA molecule comprising SEQ ID NO: 17, 14, or 16. 
     
     
         14 . A method of excising the INHT26 transgenic locus from the genome of the soybean plant cell of  claim 1  or  2 , comprising the steps of:
 (a) contacting the genome of the soybean plant cell with: (i) an RNA dependent DNA endonuclease (RdDe); and (ii) a guide RNA (gRNA) capable of hybridizing to the guide RNA hybridization site of the OgRRS and the CgRRS; wherein the RdDe recognizes a OgRRS/gRNA and a CgRRS/gRNA hybridization complex; and, 
 (b) selecting a transgenic plant cell, transgenic plant part, or transgenic plant wherein the INHT26 transgenic locus flanked by the OgRRS and the CgRRS has been excised.