Methods and compositions for repair of cartilage using an in vivo bioreactor

Abstract

Methods and compositions for the biological repair of cartilage using a hybrid construct combining both an inert structure and living core are described. The inert structure is intended to act not only as a delivery system to feed and grow a living core component, but also as an inducer of cell differentiation. The inert structure comprises concentric internal and external and inflatable/expandable balloon-like bio-polymers. The living core comprises the cell-matrix construct comprised of HDFs, for example, seeded in a scaffold. The method comprises surgically removing a damaged cartilage from a patient and inserting the hybrid construct into the cavity generated after the foregoing surgical intervention. The balloons of the inert structure are successively inflated within the target area, such as a joint, for example. Also disclosed herein are methods for growing and differentiating human fibroblasts into chondrocyte-like cells via mechanical strain.

We claim: 
     
         1 . A composition comprising chondrocyte-like cells derived from fibroblasts. 
     
     
         2 . The composition of  claim 1 , wherein said chondrocyte-like cells produce one or more of aggrecan, type II collagen, Sox-9 protein, cartilage link protein, perlecan, and sulfated proteoglycan. 
     
     
         3 . The composition of  claim 1 , wherein the fibroblasts are dermal fibroblasts, tendon fibroblasts, synovial fibroblasts, or ligament fibroblasts. 
     
     
         4 . The composition of  claim 1 , wherein the fibroblasts are autologous or allogeneic. 
     
     
         5 . The composition of  claim 1 , wherein the fibroblasts are autologous human dermal fibroblasts (HDFs) or neonatal HDFs. 
     
     
         6 . A kit comprising the composition of  claim 1 . 
     
     
         7 . The kit of  claim 6 , wherein the chondrocyte-like cells are housed in a syringe.