Systems and methods for writing, reading, and controlling data stored in a polymer

Abstract

The disclosure provides novel DNA sequences, synthesized in the 3′ to 5′ direction using topoisomerase, comprising a sequence of oligomeric cassettes, wherein each oligomeric cassette comprises a topoisomerase recognition sequence and an informational sequence, and methods for making and using the same.

1 .- 31 . (canceled) 
     
     
         32 . A DNA sequence comprising an informational series of oligomeric cassettes, wherein each oligomeric cassette comprises a topoisomerase recognition sequence and an informational sequence. 
     
     
         33 . The DNA sequence of  claim 32  wherein the topoisomerase recognition sequence is recognized by Vaccinia topoisomerase I or by SVF topoisomerase 1. 
     
     
         34 . The DNA sequence of  claim 32  wherein the topoisomerase recognition sequence is 5′-(C/T)CCTT-3′ or 5′-CCCTG-3′. 
     
     
         35 . The DNA sequence of  claim 34  wherein the topoisomerase recognition site is 5′-CCCTT-3′. 
     
     
         36 . The DNA sequence of  claim 32  wherein the informational sequence of each cassette is selected from at least two different sequences. 
     
     
         37 . The DNA sequence of  claim 36  wherein the informational sequence of each cassette is a sequence of 3 to 12 nucleotides. 
     
     
         38 . The DNA sequence of  claim 36  wherein the informational sequence of each cassette is selected from two different sequences, one of which corresponds to a “1” and one of which corresponds to a “0”, so that the informational series of oligomeric cassettes contains information in binary code form.