US2023383245A1PendingUtilityA1
Culture medium composition
Est. expiryJan 23, 2034(~7.5 yrs left)· nominal 20-yr term from priority
Inventors:Hisato HayashiMisayo OtaniKoichiro SaruhashiTaito NishinoTakehisa IwamaTatsuro KanakiAyako Aihara
C12N 2535/00C12N 2533/78C12N 2533/72C12N 2509/10A01N 1/122C12N 5/0068C12N 2533/70C12M 25/16
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Claims
Abstract
The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues, without substantially increasing the viscosity of the solution, by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.
Claims
exact text as granted — not AI-modified1 . A method of producing a cell sphere comprising adherent cells, comprising suspension culturing the adherent cells in a medium composition comprising a chitin nanofiber attached to the adherent cell, wherein the medium composition has a chitin nanofiber content of 0.001% (weight/volume) to 0.1% (weight/volume).
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