Methods and systems for chemoautotrophic production of organic compounds

Abstract

The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.

1 - 31 . (canceled) 
     
     
         32 . A host cell that comprises a heterologous polynucleotide encoding a 6-phospho-3-hexuloisomerase (PHI), wherein the polynucleotide comprises a sequence that is at least 90% identical to SEQ ID NO: 48. 
     
     
         33 . The host cell of  claim 32 , wherein the polynucleotide comprises SEQ ID NO: 48. 
     
     
         34 . The host cell of  claim 32 , wherein the host cell is a bacterial cell. 
     
     
         35 . The host cell of  claim 34 , wherein the bacterial cell is an  Escherichia coli  cell. 
     
     
         36 . The host cell of  claim 32 , wherein the host cell is a chemoautotroph. 
     
     
         37 . The host cell of  claim 32 , wherein the host cell further comprises a polynucleotide encoding a hexulose-6-phosphate synthase (HPS). 
     
     
         38 . The host cell of  claim 37 , wherein the host cell is capable of converting an inorganic carbon to a carbon-based central metabolite. 
     
     
         39 . The chemoautotrophic host cell of  claim 38 , wherein the carbon-based central metabolite is fructose-6-phosphate. 
     
     
         40 . A method for producing a carbon-based product, comprising culturing the host cell of  claim 32 . 
     
     
         41 . A polynucleotide comprising a sequence that is at least 90% identical to SEQ ID NO: 48. 
     
     
         42 . The polynucleotide of  claim 41 , wherein the polynucleotide comprises SEQ ID NO: 48. 
     
     
         43 . A vector comprising the polynucleotide of  claim 41 . 
     
     
         44 . A host cell that comprises a heterologous polynucleotide encoding a 6-phospho-3-hexuloisomerase (PHI), wherein the PHI comprises a sequence that is at least 90% identical to the  Escherichia coli  PHI protein encoded by SEQ ID NO: 48, wherein the PHI protein encoded by SEQ ID NO: 48 corresponds to NCBI Reference Sequence YP_115431. 
     
     
         45 . The host cell of  claim 44 , wherein the host cell is a bacterial cell. 
     
     
         46 . The host cell of  claim 45 , wherein the bacterial cell is an  Escherichia coli  cell. 
     
     
         47 . The host cell of  claim 44 , wherein the host cell is a chemoautotroph. 
     
     
         48 . The host cell of  claim 44 , wherein the host cell further comprises a polynucleotide encoding a hexulose-6-phosphate synthase (HPS). 
     
     
         49 . The host cell of  claim 48 , wherein the chemoautotrophic host cell is capable of converting an inorganic carbon to a carbon-based central metabolite. 
     
     
         50 . The chemoautotrophic host cell of  claim 49 , wherein the carbon-based central metabolite is fructose-6-phosphate. 
     
     
         51 . A method for producing a carbon-based product, comprising culturing the chemoautotrophic host cell of  claim 44 .