Adaptive expert system for analysis and classification of dna data

Abstract

Described herein is an adaptive expert system comprising a computing device having a memory that stores programmatic instructions and a processor that executes the programmatic instructions. The adaptive expert system receives sample data comprising at least one of raw data, optical data, and electropherogram data from a DNA analysis device, said data generated from a sample containing DNA. The adaptive expert system determines at least one characteristic of the sample data. The adaptive expert system utilizes the at least one characteristic to classify the sample data and apply a predefined parameter set to the said sample data to generate an output.

What is claimed is: 
     
         1 . An adaptive expert system comprising:
 a computing device having a memory that stores programmatic instructions and a processor that executes the programmatic instructions to:   receive sample data comprising at least one of raw data, optical data, or electropherogram data from a DNA analysis device, said data generated from a sample containing DNA;   generate a baseline DNA ID from said sample data;   determine at least one characteristic of the baseline DNA ID; and   utilize the at least one characteristic to classify the baseline DNA ID and apply a predefined parameter set to said sample data to generate an output.   
     
     
         2 . The adaptive expert system of  claim 1 , wherein the output comprises one or more of: an .xml file, an .fsa file, a .bmp file, or an allele table. 
     
     
         3 . The adaptive expert system of  claim 1 , wherein the at least one characteristic is signal strength. 
     
     
         4 . The adaptive expert system of  claim 1 , wherein the at least one characteristic is one or more of: signal to noise ratio of each dye channel, peak position, peak base assignments, peak fragment size, peak color, peak locus, peak allele #, peak height, peak width, peak shape, peak morphology, peak asymmetry, peak width deviation, peak shape deviation, base confidences, heterozygote peak height, heterozygote peak height ratio, average heterozygote peak height by color, homozygote peak height, average homozygote peak height by color, relative signal strength of alleles within loci, relative signal strength of alleles as compared to allele size (e.g. bases), iNTA peak height, iNTA peak height ratio, stutter peak height, stutter ratio, ILS success, number of peaks in the locus, number of called alleles, number of alleles labeled in red warning boxes, number of alleles unlabeled, number of loci with alleles with PH less than the PH threshold, number of loci with alleles with PH greater than PH threshold, number of loci with PHR less than PHR threshold, number of loci with PHR greater than PHR threshold, number of loci with wide peaks, number of loci with iNTA greater than iNTA threshold, number of loci with three or more peaks that are labeled in red warning boxes, number of loci with four or more peaks that are labeled in red warning boxes, number of heterozygote loci labeled in red warning boxes, number of homozygote loci labeled in red warning boxes, number of bleed-through peaks, number of CODIS20 loci called, number of CODIS 18 loci called, number of autosomal loci called, number of flex plex loci called, DNA ID success, base assignments, dye spectra, or processed dye traces. 
     
     
         5 . The adaptive expert system of  claim 1 , wherein the output is utilized to search a database of DNA IDs. 
     
     
         6 . The adaptive expert system of  claim 1 , wherein the output is utilized to compare two or more DNA IDs to assess kinship. 
     
     
         7 . The adaptive expert system of  claim 1 , wherein when the characteristic classifies the sample data as having high DNA content, the predefined parameter set applied modifies at least one of: Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Homozygote Peak Height Threshold, Heterozygote Peak Height Threshold for 2nd Peak, Homozygote Peak Height Ratio Threshold, Hemizygote Peak Height Minimum Threshold, Allele PH/PA Threshold in a first Zone, Allele PH/PA Threshold in a second Zone, Allele PH/PA Threshold in a third Zone, Allele PH/PA Threshold in a fourth Zone, Maximum Number of Dropins, Maximum Number of Dropouts, Signal to Noise Ratio Threshold of one or more dye channels, Peak Base Assignment Limits, Peak Fragment Size Threshold, Peak Height Threshold, Peak Width Threshold, Peak Shape Threshold, Peak Morphology Threshold, Peak Asymmetry Threshold, Peak Width Deviation Limit, Peak Shape Deviation Limit, Base Confidences Threshold, Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Average Heterozygote Peak Height by Color Threshold, Homozygote Peak Height Threshold, Average Homozygote Peak Height by Color Threshold, Relative Signal Strength of Alleles within Loci Threshold, Relative Signal Strength of Alleles as Compared to Allele Size Threshold, iNTA Peak Height Threshold, iNTA Peak Height Ratio Threshold, Stutter Peak Height Threshold, Stutter Ratio Threshold, Triallate Peak Height Threshold, Triallele Peak Height Ratio Threshold, Mixture Peak Height Threshold, ILS Success Limits, High iNTA Rule State, Mixture Sample Protection Rule State, Low Signal Protection Rule State, Maximum Number of Peaks in a Locus, Maximum Number of Called Alleles, Maximum Number of Alleles Labeled in Red Warning Boxes, Maximum Number of Unlabeled Alleles, Maximum Number of Loci with Alleles with PH Less than a PH Threshold, Maximum Number of Loci with Alleles with PH Greater than the PH Threshold, Maximum Number of Loci with PHR Less than a PHR Threshold, Maximum Number of Loci with PHR Greater than the PHR Threshold, Maximum Number of Loci with Wide Peaks, Maximum Number of Loci with iNTA Greater than iNTA Threshold, Maximum Number of Loci with Three or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Loci with Four or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Heterozygote Loci Labeled in Red Warning Boxes, Maximum Number of Homozygote Loci Labeled in Red Warning Boxes, Maximum Number of Bleed-through Peaks, Minimum Number of CODIS20 Loci Called, Minimum Number of CODIS18 Loci Called, Minimum Number of Autosomal Loci Called, Minimum Number of Flex Plex Loci Called, Minimum Number of Called Loci to Generate a CMF File, Quality of DNA ID Threshold, or Quality of Search and Match Threshold. 
     
     
         8 . The adaptive expert system of  claim 1 , wherein when the characteristic classifies the optical data as having low DNA content, the predefined parameter set applied modifies at least one of: Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Homozygote Peak Height Threshold, Heterozygote Peak Height Threshold for 2nd Peak, Homozygote Peak Height Ratio Threshold, Hemizygote Peak Height Minimum Threshold, Allele PH/PA Threshold in a first Zone, Allele PH/PA Threshold in a second Zone, Allele PH/PA Threshold in a third Zone, Allele PH/PA Threshold in a fourth Zone, Maximum Number of Dropins, Maximum Number of Dropouts, Signal to Noise Ratio Threshold of one or more dye channels, Peak Base Assignment Limits, Peak Fragment Size Threshold, Peak Height Threshold, Peak Width Threshold, Peak Shape Threshold, Peak Morphology Threshold, Peak Asymmetry Threshold, Peak Width Deviation Limit, Peak Shape Deviation Limit, Base Confidences Threshold, Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Average Heterozygote Peak Height by Color Threshold, Homozygote Peak Height Threshold, Average Homozygote Peak Height by Color Threshold, Relative Signal Strength of Alleles within Loci Threshold, Relative Signal Strength of Alleles as Compared to Allele Size Threshold, iNTA Peak Height Threshold, iNTA Peak Height Ratio Threshold, Stutter Peak Height Threshold, Stutter Ratio Threshold, Triallate Peak Height Threshold, Triallele Peak Height Ratio Threshold, Mixture Peak Height Threshold, ILS Success Limits, High iNTA Rule State, Mixture Sample Protection Rule State, Low Signal Protection Rule State, Maximum Number of Peaks in a Locus, Maximum Number of Called Alleles, Maximum Number of Alleles Labeled in Red Warning Boxes, Maximum Number of Unlabeled Alleles, Maximum Number of Loci with Alleles with PH Less than a PH Threshold, Maximum Number of Loci with Alleles with PH Greater than the PH Threshold, Maximum Number of Loci with PHR Less than a PHR Threshold, Maximum Number of Loci with PHR Greater than the PHR Threshold, Maximum Number of Loci with Wide Peaks, Maximum Number of Loci with iNTA Greater than iNTA Threshold, Maximum Number of Loci with Three or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Loci with Four or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Heterozygote Loci Labeled in Red Warning Boxes, Maximum Number of Homozygote Loci Labeled in Red Warning Boxes, Maximum Number of Bleed-through Peaks, Minimum Number of CODIS20 Loci Called, Minimum Number of CODIS18 Loci Called, Minimum Number of Autosomal Loci Called, Minimum Number of Flex Plex Loci Called, Minimum Number of Called Loci to Generate a CMF File, Quality of DNA ID Threshold, or Quality of Search and Match Threshold. 
     
     
         9 . The adaptive expert system of  claim 1 , wherein when the characteristic classifies the sample data as a mixture, the predefined parameter set applied modifies at least one of: Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Homozygote Peak Height Threshold, Heterozygote Peak Height Threshold for 2nd Peak, Homozygote Peak Height Ratio Threshold, Hemizygote Peak Height Minimum Threshold, Allele PH/PA Threshold in a first Zone, Allele PH/PA Threshold in a second Zone, Allele PH/PA Threshold in a third Zone, Allele PH/PA Threshold in a fourth Zone, Maximum Number of Dropins, Maximum Number of Dropouts, Signal to Noise Ratio Threshold of one or more dye channels, Peak Base Assignment Limits, Peak Fragment Size Threshold, Peak Height Threshold, Peak Width Threshold, Peak Shape Threshold, Peak Morphology Threshold, Peak Asymmetry Threshold, Peak Width Deviation Limit, Peak Shape Deviation Limit, Base Confidences Threshold, Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Average Heterozygote Peak Height by Color Threshold, Homozygote Peak Height Threshold, Average Homozygote Peak Height by Color Threshold, Relative Signal Strength of Alleles within Loci Threshold, Relative Signal Strength of Alleles as Compared to Allele Size Threshold, iNTA Peak Height Threshold, iNTA Peak Height Ratio Threshold, Stutter Peak Height Threshold, Stutter Ratio Threshold, Triallate Peak Height Threshold, Triallele Peak Height Ratio Threshold, Mixture Peak Height Threshold, ILS Success Limits, High iNTA Rule State, Mixture Sample Protection Rule State, Low Signal Protection Rule State, Maximum Number of Peaks in a Locus, Maximum Number of Called Alleles, Maximum Number of Alleles Labeled in Red Warning Boxes, Maximum Number of Unlabeled Alleles, Maximum Number of Loci with Alleles with PH Less than a PH Threshold, Maximum Number of Loci with Alleles with PH Greater than the PH Threshold, Maximum Number of Loci with PHR Less than a PHR Threshold, Maximum Number of Loci with PHR Greater than the PHR Threshold, Maximum Number of Loci with Wide Peaks, Maximum Number of Loci with iNTA Greater than iNTA Threshold, Maximum Number of Loci with Three or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Loci with Four or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Heterozygote Loci Labeled in Red Warning Boxes, Maximum Number of Homozygote Loci Labeled in Red Warning Boxes, Maximum Number of Bleed-through Peaks, Minimum Number of CODIS20 Loci Called, Minimum Number of CODIS18 Loci Called, Minimum Number of Autosomal Loci Called, Minimum Number of Flex Plex Loci Called, Minimum Number of Called Loci to Generate a CMF File, Quality of DNA ID Threshold, or Quality of Search and Match Threshold. 
     
     
         10 . The adaptive expert system of  claim 9 , in which the applied predefined parameter set generates a first set of called alleles, and said called alleles are extracted from the sample data generating modified sample data, and the adaptive expert system utilizes the at least one characteristic to classify the modified sample data and apply a predefined parameter set to said modified sample data to generate a second set of called alleles. 
     
     
         11 . The adaptive expert system of  claim 1 , wherein when the characteristic classifies the sample data as inhibited and/or degraded, the predefined parameter set applied modifies at least one of: Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Homozygote Peak Height Threshold, Heterozygote Peak Height Threshold for 2nd Peak, Homozygote Peak Height Ratio Threshold, Hemizygote Peak Height Minimum Threshold, Allele PH/PA Threshold in a first Zone, Allele PH/PA Threshold in a second Zone, Allele PH/PA Threshold in a third Zone, Allele PH/PA Threshold in a fourth Zone, Maximum Number of Dropins, Maximum Number of Dropouts, Signal to Noise Ratio Threshold of one or more dye channels, Peak Base Assignment Limits, Peak Fragment Size Threshold, Peak Height Threshold, Peak Width Threshold, Peak Shape Threshold, Peak Morphology Threshold, Peak Asymmetry Threshold, Peak Width Deviation Limit, Peak Shape Deviation Limit, Base Confidences Threshold, Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Average Heterozygote Peak Height by Color Threshold, Homozygote Peak Height Threshold, Average Homozygote Peak Height by Color Threshold, Relative Signal Strength of Alleles within Loci Threshold, Relative Signal Strength of Alleles as Compared to Allele Size Threshold, iNTA Peak Height Threshold, iNTA Peak Height Ratio Threshold, Stutter Peak Height Threshold, Stutter Ratio Threshold, Triallate Peak Height Threshold, Triallele Peak Height Ratio Threshold, Mixture Peak Height Threshold, ILS Success Limits, High iNTA Rule State, Mixture Sample Protection Rule State, Low Signal Protection Rule State, Maximum Number of Peaks in a Locus, Maximum Number of Called Alleles, Maximum Number of Alleles Labeled in Red Warning Boxes, Maximum Number of Unlabeled Alleles, Maximum Number of Loci with Alleles with PH Less than a PH Threshold, Maximum Number of Loci with Alleles with PH Greater than the PH Threshold, Maximum Number of Loci with PHR Less than a PHR Threshold, Maximum Number of Loci with PHR Greater than the PHR Threshold, Maximum Number of Loci with Wide Peaks, Maximum Number of Loci with iNTA Greater than iNTA Threshold, Maximum Number of Loci with Three or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Loci with Four or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Heterozygote Loci Labeled in Red Warning Boxes, Maximum Number of Homozygote Loci Labeled in Red Warning Boxes, Maximum Number of Bleed-through Peaks, Minimum Number of CODIS20 Loci Called, Minimum Number of CODIS18 Loci Called, Minimum Number of Autosomal Loci Called, Minimum Number of Flex Plex Loci Called, Minimum Number of Called Loci to Generate a CMF File, Quality of DNA ID Threshold, or Quality of Search and Match Threshold. 
     
     
         12 . The adaptive expert system of  claim 1 , wherein based upon classifying the baseline DNA ID and applying a predefined parameter set, the computing device modifies a value of one or more characteristics utilized to classify a DNA ID and apply a predefined parameter set. 
     
     
         13 . The adaptive expert system of  claim 1 , wherein based upon classifying the baseline DNA ID and applying a predefined parameter set, the computing device modifies one or more predefined parameter sets utilized to generate an output when applied to a DNA ID. 
     
     
         14 . The adaptive expert system of  claim 1 , wherein the computing device generates a visualization of the output for presentation on a display device. 
     
     
         15 . The adaptive expert system of  claim 14 , wherein the visualization comprises a number of alleles called for each of one or more parameter sets. 
     
     
         16 . An adaptive expert system comprising:
 a computing device having a memory that stores programmatic instructions and a processor that executes the programmatic instructions to:   receive data from an analysis device, said data generated from a sample containing an analyte;   determine, using the data, at least one characteristic of the analyte; and   utilize the at least one characteristic to classify the data and apply a predefined parameter set to the data to generate an output.   
     
     
         17 . The system of  claim 16 , wherein the analyte is one or more of: proteins, peptides, messenger RNAs, antisense RNAs, transfer RNAs, ribosomal RNAs, other RNAs, nucleic acids, oligonucleotides, DNA or RNA fragments containing Single Nucleotide Polymorphisms, lipids, carbohydrates, metabolites, steroids, synthetic polymers, other macromolecules, inorganic or other organic chemicals, or combinations of these analytes. 
     
     
         18 . An adaptive expert system comprising:
 a computing device having a memory that stores programmatic instructions and a processor that executes the programmatic instructions to:   receive data from a DNA analysis device, said data generated from a sample containing a plurality of genomic DNA from at least one major contributor and at least one minor contributor;   determine, using the data, at least one characteristic of a first DNA ID corresponding to a major contributor;   utilize the characteristic to classify the first DNA ID and apply a predefined parameter set to the data;   subtract one or more characteristics from the first DNA ID to determine at least one characteristic of a second DNA ID corresponding to a minor contributor.   
     
     
         19 . An adaptive expert system comprising:
 a computing device having a set of decision making nodes, said nodes capable of self-learning when presented with a set of sample data with known outputs;   said computing device having a memory that stores programmatic instructions and a processor that executes the programmatic instructions to:   receive sample data comprising at least one of raw data, optical data, and electropherogram data from a DNA analysis device, said data generated from a sample containing DNA;   determine at least one characteristic of the sample data;   apply said set of decision making nodes to generate an optimal set of expert system parameters for a given set of characteristics from the sample data; and apply the optimal set of expert system parameters to the said sample data to generate an output   
     
     
         20 . The adaptive expert system of  claim 19 , wherein the output comprises one or more of: an .xml file, an .fsa file, a .bmp file, or an allele table. 
     
     
         21 . The adaptive expert system of  claim 19 , wherein the at least one characteristic is signal strength. 
     
     
         22 . The adaptive expert system of  claim 19 , wherein the at least one characteristic is one or more of: signal to noise ratio of each dye channel, peak position, peak base assignments, peak fragment size, peak color, peak locus, peak allele #, peak height, peak width, peak shape, peak morphology, peak asymmetry, peak width deviation, peak shape deviation, base confidences, heterozygote peak height, heterozygote peak height ratio, average heterozygote peak height by color, homozygote peak height, average homozygote peak height by color, relative signal strength of alleles within loci, relative signal strength of alleles as compared to allele size (e.g. bases), iNTA peak height, iNTA peak height ratio, stutter peak height, stutter ratio, ILS success, number of peaks in the locus, number of called alleles, number of alleles labeled in red warning boxes, number of alleles unlabeled, number of loci with alleles with PH less than the PH threshold, number of loci with alleles with PH greater than PH threshold, number of loci with PHR less than PHR threshold, number of loci with PHR greater than PHR threshold, number of loci with wide peaks, number of loci with iNTA greater than iNTA threshold, number of loci with three or more peaks that are labeled in red warning boxes, number of loci with four or more peaks that are labeled in red warning boxes, number of heterozygote loci labeled in red warning boxes, number of homozygote loci labeled in red warning boxes, number of bleed-through peaks, number of CODIS20 loci called, number of CODIS 18 loci called, number of autosomal loci called, number of flex plex loci called, DNA ID success, base assignments, dye spectra, or processed dye traces. 
     
     
         23 . The adaptive expert system of  claim 19 , wherein the output is utilized to search a database of DNA IDs. 
     
     
         24 . The adaptive expert system of  claim 19 , wherein the output is utilized to compare two or more DNA IDs to assess kinship. 
     
     
         25 . The adaptive expert system of  claim 19 , wherein when the characteristic classifies the sample data as having high DNA content, the predefined parameter set applied modifies at least one of: Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Homozygote Peak Height Threshold, Heterozygote Peak Height Threshold for 2nd Peak, Homozygote Peak Height Ratio Threshold, Hemizygote Peak Height Minimum Threshold, Allele PH/PA Threshold in a first Zone, Allele PH/PA Threshold in a second Zone, Allele PH/PA Threshold in a third Zone, Allele PH/PA Threshold in a fourth Zone, Maximum Number of Dropins, Maximum Number of Dropouts, Signal to Noise Ratio Threshold of one or more dye channels, Peak Base Assignment Limits, Peak Fragment Size Threshold, Peak Height Threshold, Peak Width Threshold, Peak Shape Threshold, Peak Morphology Threshold, Peak Asymmetry Threshold, Peak Width Deviation Limit, Peak Shape Deviation Limit, Base Confidences Threshold, Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Average Heterozygote Peak Height by Color Threshold, Homozygote Peak Height Threshold, Average Homozygote Peak Height by Color Threshold, Relative Signal Strength of Alleles within Loci Threshold, Relative Signal Strength of Alleles as Compared to Allele Size Threshold, iNTA Peak Height Threshold, iNTA Peak Height Ratio Threshold, Stutter Peak Height Threshold, Stutter Ratio Threshold, Triallate Peak Height Threshold, Triallele Peak Height Ratio Threshold, Mixture Peak Height Threshold, ILS Success Limits, High iNTA Rule State, Mixture Sample Protection Rule State, Low Signal Protection Rule State, Maximum Number of Peaks in a Locus, Maximum Number of Called Alleles, Maximum Number of Alleles Labeled in Red Warning Boxes, Maximum Number of Unlabeled Alleles, Maximum Number of Loci with Alleles with PH Less than a PH Threshold, Maximum Number of Loci with Alleles with PH Greater than the PH Threshold, Maximum Number of Loci with PHR Less than a PHR Threshold, Maximum Number of Loci with PHR Greater than the PHR Threshold, Maximum Number of Loci with Wide Peaks, Maximum Number of Loci with iNTA Greater than iNTA Threshold, Maximum Number of Loci with Three or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Loci with Four or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Heterozygote Loci Labeled in Red Warning Boxes, Maximum Number of Homozygote Loci Labeled in Red Warning Boxes, Maximum Number of Bleed-through Peaks, Minimum Number of CODIS20 Loci Called, Minimum Number of CODIS18 Loci Called, Minimum Number of Autosomal Loci Called, Minimum Number of Flex Plex Loci Called, Minimum Number of Called Loci to Generate a CMF File, Quality of DNA ID Threshold, or Quality of Search and Match Threshold. 
     
     
         26 . The adaptive expert system of  claim 19 , wherein when the characteristic classifies the sample data as having low DNA content, the predefined parameter set applied modifies at least one of: Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Homozygote Peak Height Threshold, Heterozygote Peak Height Threshold for 2nd Peak, Homozygote Peak Height Ratio Threshold, Hemizygote Peak Height Minimum Threshold, Allele PH/PA Threshold in a first Zone, Allele PH/PA Threshold in a second Zone, Allele PH/PA Threshold in a third Zone, Allele PH/PA Threshold in a fourth Zone, Maximum Number of Dropins, Maximum Number of Dropouts, Signal to Noise Ratio Threshold of one or more dye channels, Peak Base Assignment Limits, Peak Fragment Size Threshold, Peak Height Threshold, Peak Width Threshold, Peak Shape Threshold, Peak Morphology Threshold, Peak Asymmetry Threshold, Peak Width Deviation Limit, Peak Shape Deviation Limit, Base Confidences Threshold, Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Average Heterozygote Peak Height by Color Threshold, Homozygote Peak Height Threshold, Average Homozygote Peak Height by Color Threshold, Relative Signal Strength of Alleles within Loci Threshold, Relative Signal Strength of Alleles as Compared to Allele Size Threshold, iNTA Peak Height Threshold, iNTA Peak Height Ratio Threshold, Stutter Peak Height Threshold, Stutter Ratio Threshold, Triallate Peak Height Threshold, Triallele Peak Height Ratio Threshold, Mixture Peak Height Threshold, ILS Success Limits, High iNTA Rule State, Mixture Sample Protection Rule State, Low Signal Protection Rule State, Maximum Number of Peaks in a Locus, Maximum Number of Called Alleles, Maximum Number of Alleles Labeled in Red Warning Boxes, Maximum Number of Unlabeled Alleles, Maximum Number of Loci with Alleles with PH Less than a PH Threshold, Maximum Number of Loci with Alleles with PH Greater than the PH Threshold, Maximum Number of Loci with PHR Less than a PHR Threshold, Maximum Number of Loci with PHR Greater than the PHR Threshold, Maximum Number of Loci with Wide Peaks, Maximum Number of Loci with iNTA Greater than iNTA Threshold, Maximum Number of Loci with Three or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Loci with Four or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Heterozygote Loci Labeled in Red Warning Boxes, Maximum Number of Homozygote Loci Labeled in Red Warning Boxes, Maximum Number of Bleed-through Peaks, Minimum Number of CODIS20 Loci Called, Minimum Number of CODIS18 Loci Called, Minimum Number of Autosomal Loci Called, Minimum Number of Flex Plex Loci Called, Minimum Number of Called Loci to Generate a CMF File, Quality of DNA ID Threshold, or Quality of Search and Match Threshold. 
     
     
         27 . The adaptive expert system of  claim 19 , wherein when the characteristic classifies the sample data as a mixture, the predefined parameter set applied modifies at least one of: Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Homozygote Peak Height Threshold, Heterozygote Peak Height Threshold for 2nd Peak, Homozygote Peak Height Ratio Threshold, Hemizygote Peak Height Minimum Threshold, Allele PH/PA Threshold in a first Zone, Allele PH/PA Threshold in a second Zone, Allele PH/PA Threshold in a third Zone, Allele PH/PA Threshold in a fourth Zone, Maximum Number of Dropins, Maximum Number of Dropouts, Signal to Noise Ratio Threshold of one or more dye channels, Peak Base Assignment Limits, Peak Fragment Size Threshold, Peak Height Threshold, Peak Width Threshold, Peak Shape Threshold, Peak Morphology Threshold, Peak Asymmetry Threshold, Peak Width Deviation Limit, Peak Shape Deviation Limit, Base Confidences Threshold, Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Average Heterozygote Peak Height by Color Threshold, Homozygote Peak Height Threshold, Average Homozygote Peak Height by Color Threshold, Relative Signal Strength of Alleles within Loci Threshold, Relative Signal Strength of Alleles as Compared to Allele Size Threshold, iNTA Peak Height Threshold, iNTA Peak Height Ratio Threshold, Stutter Peak Height Threshold, Stutter Ratio Threshold, Triallate Peak Height Threshold, Triallele Peak Height Ratio Threshold, Mixture Peak Height Threshold, ILS Success Limits, High iNTA Rule State, Mixture Sample Protection Rule State, Low Signal Protection Rule State, Maximum Number of Peaks in a Locus, Maximum Number of Called Alleles, Maximum Number of Alleles Labeled in Red Warning Boxes, Maximum Number of Unlabeled Alleles, Maximum Number of Loci with Alleles with PH Less than a PH Threshold, Maximum Number of Loci with Alleles with PH Greater than the PH Threshold, Maximum Number of Loci with PHR Less than a PHR Threshold, Maximum Number of Loci with PHR Greater than the PHR Threshold, Maximum Number of Loci with Wide Peaks, Maximum Number of Loci with iNTA Greater than iNTA Threshold, Maximum Number of Loci with Three or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Loci with Four or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Heterozygote Loci Labeled in Red Warning Boxes, Maximum Number of Homozygote Loci Labeled in Red Warning Boxes, Maximum Number of Bleed-through Peaks, Minimum Number of CODIS20 Loci Called, Minimum Number of CODIS18 Loci Called, Minimum Number of Autosomal Loci Called, Minimum Number of Flex Plex Loci Called, Minimum Number of Called Loci to Generate a CMF File, Quality of DNA ID Threshold, or Quality of Search and Match Threshold. 
     
     
         28 . The adaptive expert system of  claim 27 , in which the applied predefined parameter set generates a first set of called alleles, and said called alleles are extracted from the sample data generating modified sample data, and the adaptive expert system utilizes the at least one characteristic to classify the modified sample data and apply a predefined parameter set to said modified sample data to generate a second set of called alleles. 
     
     
         29 . The adaptive expert system of  claim 19 , wherein when the characteristic classifies the sample data as inhibited and/or degraded, the predefined parameter set applied modifies at least one of: Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Homozygote Peak Height Threshold, Heterozygote Peak Height Threshold for 2nd Peak, Homozygote Peak Height Ratio Threshold, Hemizygote Peak Height Minimum Threshold, Allele PH/PA Threshold in a first Zone, Allele PH/PA Threshold in a second Zone, Allele PH/PA Threshold in a third Zone, Allele PH/PA Threshold in a fourth Zone, Maximum Number of Dropins, Maximum Number of Dropouts, Signal to Noise Ratio Threshold of one or more dye channels, Peak Base Assignment Limits, Peak Fragment Size Threshold, Peak Height Threshold, Peak Width Threshold, Peak Shape Threshold, Peak Morphology Threshold, Peak Asymmetry Threshold, Peak Width Deviation Limit, Peak Shape Deviation Limit, Base Confidences Threshold, Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Average Heterozygote Peak Height by Color Threshold, Homozygote Peak Height Threshold, Average Homozygote Peak Height by Color Threshold, Relative Signal Strength of Alleles within Loci Threshold, Relative Signal Strength of Alleles as Compared to Allele Size Threshold, iNTA Peak Height Threshold, iNTA Peak Height Ratio Threshold, Stutter Peak Height Threshold, Stutter Ratio Threshold, Triallate Peak Height Threshold, Triallele Peak Height Ratio Threshold, Mixture Peak Height Threshold, ILS Success Limits, High iNTA Rule State, Mixture Sample Protection Rule State, Low Signal Protection Rule State, Maximum Number of Peaks in a Locus, Maximum Number of Called Alleles, Maximum Number of Alleles Labeled in Red Warning Boxes, Maximum Number of Unlabeled Alleles, Maximum Number of Loci with Alleles with PH Less than a PH Threshold, Maximum Number of Loci with Alleles with PH Greater than the PH Threshold, Maximum Number of Loci with PHR Less than a PHR Threshold, Maximum Number of Loci with PHR Greater than the PHR Threshold, Maximum Number of Loci with Wide Peaks, Maximum Number of Loci with iNTA Greater than iNTA Threshold, Maximum Number of Loci with Three or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Loci with Four or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Heterozygote Loci Labeled in Red Warning Boxes, Maximum Number of Homozygote Loci Labeled in Red Warning Boxes, Maximum Number of Bleed-through Peaks, Minimum Number of CODIS20 Loci Called, Minimum Number of CODIS18 Loci Called, Minimum Number of Autosomal Loci Called, Minimum Number of Flex Plex Loci Called, Minimum Number of Called Loci to Generate a CMF File, Quality of DNA ID Threshold, or Quality of Search and Match Threshold. 
     
     
         30 . The adaptive expert system of  claim 19 , wherein based upon classifying the sample data and applying a predefined parameter set, the computing device modifies a value of one or more characteristics utilized to classify the sample data and apply a predefined parameter set. 
     
     
         31 . The adaptive expert system of  claim 19 , wherein based upon classifying the sample data and applying a predefined parameter set, the computing device modifies one or more predefined parameter sets utilized to generate an output when applied to a DNA ID. 
     
     
         32 . The adaptive expert system of  claim 19 , wherein the computing device generates a visualization of the output for presentation on a display device. 
     
     
         33 . The adaptive expert system of  claim 32 , wherein the visualization comprises a number of alleles called for each of one or more parameter sets. 
     
     
         34 . An adaptive expert system comprising:
 a computing device having a memory that stores programmatic instructions and a processor that executes the programmatic instructions to:   receive sample data from an optical detection system comprising at least two detectors or detector elements, said detectors or detector elements configured to collect fluorescence from dye-labelled DNA to generate an optical signal;   subtract a baseline of the optical signal;   where for at least one peak in the sample data, saturate the optical signal of at least one of the detectors or detector elements, and not saturate the optical signal of at least one of the detectors or detector elements;   calculate signal strength ratios of the detector or detector element signal of each of the saturated detectors or detector elements to each of the unsaturated detectors or detector elements in a portion of the peak where both detectors or detector elements are not saturated; and   calculate signal strength of the saturated detectors or detector elements in the saturated portion of the peak by multiplying signal strength of the unsaturated detectors or detector elements by the corresponding signal strength ratio.   
     
     
         35 . The adaptive expert system of  claim 34 , wherein the optical detection system comprises at least 4 detectors or detector elements. 
     
     
         36 . The adaptive expert system of  claim 35 , wherein the optical detection system comprises at least 5 detectors or detector elements. 
     
     
         37 . The adaptive expert system of  claim 36 , wherein the optical detection system comprises at least 6 detectors or detector elements. 
     
     
         38 . The adaptive expert system of  claim 37 , wherein the optical detection system comprises at least 8 detectors or detector elements. 
     
     
         39 . The adaptive expert system of  claim 38 , wherein the optical detection system comprises at least 12 detectors or detector elements. 
     
     
         40 . The adaptive expert system of  claim 39 , wherein the optical detection system comprises at least 16 detectors or detector elements. 
     
     
         41 . The adaptive expert system of  claim 40 , wherein the optical detection system comprises at least 32 detectors or detector elements. 
     
     
         42 . The adaptive expert system of  claim 41 , wherein the optical detection system comprises at least 64 detectors or detector elements. 
     
     
         43 . The adaptive expert system of claim  424 , wherein the optical detection system comprises at least 128 detectors or detector elements. 
     
     
         44 . The adaptive expert system of  claim 43 , wherein the optical detection system comprises at least 256 detectors or detector elements. 
     
     
         45 . An adaptive expert system comprising:
 a computing device having a memory that stores programmatic instructions and a processor that executes the programmatic instructions to:   receive sample data comprising at least one of raw data, optical data, and electropherogram data from a DNA analysis device, said data generated from a sample containing DNA;   determine at least one criteria for maximizing results of an iteration; and   modify at least one parameter by iteratively varying the parameter value in order to maximize the criteria, and generate an output.   
     
     
         46 . The adaptive expert system of  claim 45 , wherein when the parameter set applied modifies at least one of: Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Homozygote Peak Height Threshold, Heterozygote Peak Height Threshold for 2nd Peak, Homozygote Peak Height Ratio Threshold, Hemizygote Peak Height Minimum Threshold, Allele PH/PA Threshold in a first Zone, Allele PH/PA Threshold in a second Zone, Allele PH/PA Threshold in a third Zone, Allele PH/PA Threshold in a fourth Zone, Maximum Number of Dropins, Maximum Number of Dropouts, Signal to Noise Ratio Threshold of one or more dye channels, Peak Base Assignment Limits, Peak Fragment Size Threshold, Peak Height Threshold, Peak Width Threshold, Peak Shape Threshold, Peak Morphology Threshold, Peak Asymmetry Threshold, Peak Width Deviation Limit, Peak Shape Deviation Limit, Base Confidences Threshold, Heterozygote Peak Height Threshold, Heterozygote Peak Height Ratio Threshold, Average Heterozygote Peak Height by Color Threshold, Homozygote Peak Height Threshold, Average Homozygote Peak Height by Color Threshold, Relative Signal Strength of Alleles within Loci Threshold, Relative Signal Strength of Alleles as Compared to Allele Size Threshold, iNTA Peak Height Threshold, iNTA Peak Height Ratio Threshold, Stutter Peak Height Threshold, Stutter Ratio Threshold, Triallate Peak Height Threshold, Triallele Peak Height Ratio Threshold, Mixture Peak Height Threshold, ILS Success Limits, High iNTA Rule State, Mixture Sample Protection Rule State, Low Signal Protection Rule State, Maximum Number of Peaks in a Locus, Maximum Number of Called Alleles, Maximum Number of Alleles Labeled in Red Warning Boxes, Maximum Number of Unlabeled Alleles, Maximum Number of Loci with Alleles with PH Less than a PH Threshold, Maximum Number of Loci with Alleles with PH Greater than the PH Threshold, Maximum Number of Loci with PHR Less than a PHR Threshold, Maximum Number of Loci with PHR Greater than the PHR Threshold, Maximum Number of Loci with Wide Peaks, Maximum Number of Loci with iNTA Greater than iNTA Threshold, Maximum Number of Loci with Three or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Loci with Four or More Peaks that are Labeled in Red Warning Boxes, Maximum Number of Heterozygote Loci Labeled in Red Warning Boxes, Maximum Number of Homozygote Loci Labeled in Red Warning Boxes, Maximum Number of Bleed-through Peaks, Minimum Number of CODIS20 Loci Called, Minimum Number of CODIS18 Loci Called, Minimum Number of Autosomal Loci Called, Minimum Number of Flex Plex Loci Called, Minimum Number of Called Loci to Generate a CMF File, Quality of DNA ID Threshold, Quality of Search and Match Threshold, or search stringency. 
     
     
         47 . The adaptive expert system of  claim 45 , wherein when the criteria for iteration is defined by at least one of: Number of called loci CODIS20, Number of called loci CODIS18, Number of called loci FlexPlex, Number of loci that change from homozygote to heterzygote, Number of dropins, Number of dropouts, Number of alleles labeled in red warning boxes, Quality of DNA ID threshold, or Quality of search and match threshold. 
     
     
         48 . The adaptive expert system of  claim 45 , wherein the steps for the iteration are not fixed and can vary depending on rate of change in the criteria.